Contributions to baseline fluorescence by the growth medium (e.g., esterase activity, proteins interacting with receptors of interest, or phenol red) are eliminated by removing the medium prior to adding the indicator dye to the wells. A ...
长波 Rhod-2 Ca2+ 指示剂是 Fluo-3 的重要替代品，适用于在具有高水平自发荧光的细胞和组织中进行实验。 Rhod-2 AM 是 Rhod-2 的细胞渗透性版本。图1.Rhod-2 AM化学结构 Rhod-2 AM基础信息 产品名：Rhod-2 AM;Cell-permeable fluorescent Ca2+ indicator; fluorescent Ca2+ indicator CAS号：145037-81-6 ...
Indo-1 AM是一种荧光 Ca2+指示剂，荧光 :λex 330 nm，λem 450 nm in 含钙； λex 356 nm，λem 478 nm 甲醇溶液。图1.Indo-1 AM化学结构 Indo-1 AM基础信息 中文别名：Indo-1 AM钙荧光探针;英文别名：Indo-1 AM, fluorescent Ca2+ indicator CAS号：112926-02-0 结构式：O=C(C1=CC2=C(C=...
Visualizing the activity of nerve cells using genetically encoded indicator proteins has emerged to a widely used technique in the field of neuroscience. In particular, intracellular Ca 2+ dynamics represents a parameter that is closely correlated with neuronal excitation, and a variety of genetically ...
M.Wt: 1009.91 Formula: C47H51N3O22 Purity: ≥90% Storage: Store at -20°C CAS No: 112926-02-0 The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis. Tocris products are intended for laboratory research use only, unless stat...
Briefly, cells were loaded with the ratiometric fluorescent Ca2+ indicator Fura2/AM (1 µM) at RT for 30 min in a modified Krebs solution (containing 150 mM NaCl, 5.9 mM KCl, 1.2 mM MgCl2, 11.6 mM HEPES (pH 7.3), and 1.5 mM CaCl2). Cells were allowed to rest...
positioning the channel at the crossroads of inflammation and tissue homeostasis. To define the function of TRPM7 in phagosome maturation, we used mouse lines that express genetically encoded Ca2+indicator GCaMP6s in myeloid cells and measured the Ca2+-elevations associated with efferocytosis inTrpm7...
The oxidation ratio of C11 BODIPY 581/591 was calculated as an indicator of lipid peroxidation per cell following the equation: $${\mathrm{Oxidation}}\;{\mathrm{ratio}} = {\mathrm{Bodipy}}^{{\mathrm{ox}}}/({\mathrm{Bodipy}}^{{\mathrm{ox}}} + {\mathrm{Bodipy}}^{{\mathrm{red}}...
The fluorescent ratiometric Indo-1 Ca2+ indicator dye was used to monitor changes in [Ca2+]cyt resulting from ER Ca2+ release and extracellular-derived influx. First, baseline recordings of [Ca2+]cyt were performed in the absence of extracellular Ca2+. This was followed by the addition of 3...
Quantifying the magnitude of Ca2+signals from changes in the emission of fluorescent indicators relies on assumptions about the indicator behaviour in situ... D Thomas,SC Tovey,TJ Collins,... - 《Cell Calcium》 被引量: 733发表: 2000年 Practical aspects of measuring [Ca2+] with fluorescent ind...