Genetically encoded Ca2+ indicator (GECIImagingVisualizing the activity of nerve cells using genetically encoded indicator proteins has emerged to a widely used technique in the field of neuroscience. In particular, intracellular Ca 2+ dynamics represents a parameter that is closely correlated with ...
What limitations were observed with the expression of other spectrally unique Genetically Encoded Ca2+ Indicators (GECIs) in filamentous fungi? What were the results of comparing Ca2+ signatures between GCaMP5G and YC3.60 in the mutants? Why is there a need for Genetically Encoded Ca2+ Indicators...
However, direct visualization of nanodomain Ca2 + far exceeds optical resolution of spatially distributed Ca2 + indicators. Here we couple an optical, genetically encoded Ca2 + indicator (TN–XL) to the carboxy tail of CaV2.2 Ca2 + channels, enabling near-field imaging ...
Split-intein mediated re-assembly of genetically encoded Ca(2+) indicators. While genetically encoded Ca2+ indicators (GECIs) allow Ca2+ imaging in model organisms, the gene expression is often under the control of a single promote... SSC Wong,I Kotera,E Mills,... - 《Cell Calcium》 被...
Recently developed genetically encoded Ca2+ indicators (GECIs) are defined as optical Ca2+ sensors produced by the expression of specific genes. These proteins contain a light-emitting fluorescent protein unit and a Ca2+-responsive unit. Troponeon, a typical member of the GECI family is composed...
An expanded palette of genetically encoded Ca2+ indicators. Science 333, 1888–1891 (2011). Article ADS CAS PubMed PubMed Central Google Scholar Shen, Y., Rosendale, M., Campbell, R. E. & Perrais, D. pHuji, a pH-sensitive red fluorescent protein for imaging of exo- and endocytosis....
Simultaneous fMRI and electrophysiological recordings offered the first insights into the NVC underlying the cortical fMRI signal in both task-related and resting-state conditions19,20. Lately, genetically encoded Ca2+indicators, for example, GCaMP, mediating Ca2+imaging from neurons or astrocytes have ...
We report an intensiometric, near-infrared fluorescent, genetically encoded calcium ion (Ca2+) indicator (GECI) with excitation and emission maxima at 678 and 704 nm, respectively. This GECI, designated NIR-GECO1, enables imaging of Ca2+ transients in cultured mammalian cells and brain tiss...
Genetically encoded calcium indicator (GECI) polypeptides and the nucleic acid molecules encoding such polypeptides are provided.
WO2011056975A22011-05-12GENETICALLY ENCODED CALCIUM INDICATORS AND METHODS OF USE Other References: Airan et al., “Temporally precise in vivo control of intracellular signaling,” Nature, 2009, 458(7241):1025-1029. Akerboom et al., “Crystal Structures of the GCaMP Calcium Sensor Reveal the...