本课题拟 给予大鼠氯化镧饮水染毒,比较不同剂量组染毒大鼠空间学习记忆能力,海马神 经细胞中Ca2+含量,ERKl/2蛋白表达水平, 磷酸化ERKl/2和磷酸化CREB蛋 白表达水及BDNF的基因转录与蛋白表达水平,探讨镧对Ca2+一ERKl/2一CREB 信号通路的影响并明确该通路在损害学习记忆中的作用,为深入阐明镧的神经毒 作用机制...
要点7:分析CD4+和CD8+ T细胞在TCR作用下的增殖潜力以及CD19+ B细胞在BCR作用下的增殖潜力来评估淋巴细胞激活的下游结果。结果表明P2的免疫反应受损,特别是对TCR的刺激,在杂合亲本中观察到微弱的影响,这与Erk的钙通量和磷酸化缺陷一致。 全文小结 FULL-TEXT SUMMARY + + + + + + + + + + + 综上所述,IT...
5. Yang KY, Zhao S, Feng H, Shen J, Chen Y, Wang ST, et al. Ca(2+) homeostasis maintained by TMCO1 underlies corpus callosum development via ERK signaling. Cell Death Dis. 2022; 13:674-86. 6. Zhong W, Wang X, Yang L, Wang Y, Xiao Q, Yu S, et al. Nanocarrier-Assisted D...
目的 探讨高浓度苯丙氨酸(Phe)对体外培养的胚鼠皮层神经元突起生长,胞内Ca2+水平和细胞外信号调节激酶(ERK)活性的影响.方法 根据加入Phe的浓度,将体外培养的原代胚鼠皮层神经元随机分为5组:0.45,0.9,3.6,5.0mmol/L Phe组和对照组(加PBS).采用细胞免疫化学方法 检测各组神经元最长突起长度;激光共聚焦显微镜下...
RASGRP1是RAS-MAPK-ERK通路上的主要激活剂之一。 KEGG RASGRP1缺陷患者发生CID,T细胞数量减少,特别是幼稚T细胞,同时ERK激活水平降低,无法响应TCR的增殖信号。这类患者会反复病毒感染,多数有严重的EBV相关性淋巴细胞增殖疾病。 PIK3信号缺陷 PI3K-AKT-mTOR参与调节T细胞分化、增殖、效应、记忆等众多过程。Ⅰ类PI3K的催化亚...
在目前的研究中,我们使用CHO细胞系,发现随着GPR40介导的钙的增加,黄连素以剂量依赖性方式活化ERK1/2和AMPK.此外,为了研究黄连素降血糖效果,我们采用腹腔注射葡萄糖和黄连素,发现黄连素较GPR40内源性配体亚油酸更显著的降低血糖浓度.总之,我们的研究可能为黄连素通过GPR40诱导ERK1/2和AMPK磷酸化提供新的药理作用和生理...
钙敏感受体基因多态性E942K通过Ca~(2+)和ERK1/2通路促进胃癌细胞增殖 本研究旨在探究钙敏感受体(calcium-sensing receptor, CaSR)的单核苷酸多态性(single nucleotide polymorphism, SNP)E942K对胃癌细胞增殖的影响及其机制.对46例胃癌患... 张亚博,杜超,卢骋,... - 《生理学报》 被引量: 0发表: 2020年研...
To further determine the relationship between extracellular signal-regulated kinase (ERK) pathway and [Ca2+]i, cells were, respectively, pretreated with PD98059 (inhibitor of ERK) or BAPTA-AM. As shown in Fig. 8, the use of BAPTA-AM to inhibit [Ca2+]i successfully increased [Ca2+]i the...
6A, B). Cells that responded showed comparable phosphorylation of Erk (Fig. 6C), indicating that the defect was in triggering pathway activation. Stimulation of the BCR resulted in relatively normal pErk positivity in B cells (Fig. 6D–F). These results suggest a functional defect of IP3R3 ...
A striking example of this was reported by the Lefkowitz lab who observed that the PTX-dependence of β2AR-mediated activation of ERK was highly variable in HEK293 cells grown by different individuals within the lab from completely PTX sensitive to completely insensitive (49). Thus, while Gβ...