Construction of tagged transformant and replacement of PbpfA The nucleotide sequences encoding tags were inserted into the corresponding location of the target gene in the genome. To construct a C-terminal 3×Flag-tagged BpfD transformant, the 3′-terminus region of the bpfD gene, including ...
cloned into the pQCXIN retroviral vector at NotI and PacI sites. APP C-terminal fragment C99- and C83 (Flag-C99 and Flag-C83, respectively) were amplified by PCR using previously described pCAGOSF-APP77016as template. APP C-terminal fragment C83 with V5-tag on the C-terminus (C83-V5) wa...
It uses the variable XD_TERM to decide which terminal program to run. If XD_TERM is unset, it uses hpterm on HP-UX and xterm on anything else. You can add other editors to the xd_edit script. This is explained, along with all the other ways in which the editing mechanism can be ...
Full-length (FL) and the N-terminal domain (NTD) of USP1 are labeled. (D) Physical interaction between GST-tagged BC-box proteins and GFP-tagged WT or mutant USP1-NTD. (E) Stability analysis of various forms of USP1 in HEK293T cells. FL-C90S is a full-length USP1 mutant that ...
When the tcsh shell's input is not a terminal, the character '#' is taken to begin a comment. Each # and the rest of the input line on which it appears is discarded before further parsing. A special character (including a blank or tab) can be prevented from having its special meaning...
# sysnameSwitch1 # vlan batch 10 # interfaceGigabitEthernet1/0/0port link-type hybridport hybrid tagged vlan 10 # interfaceGigabitEthernet2/0/0port link-type hybridport hybrid tagged vlan 10 # return PE1 configuration file # sysname PE1 # router id 1.1.1.1...
it could modify the pointer itself or the pointee object. such cases are already being tagged by 12.13 and the fix to that rule will either make the source code in a way that will be tagged by 16.7 or fix it so it wont get tagged. Either way, it is pointless to look for changes ...
FLAG-tagged CtBP2 was expressed in COS-7 cells in the absence (A and C) and presence (B and D) of transfected HIPK2. FLAG-CtBP2 was immunoprecipitated and subjected to SDS-PAGE and tryptic digest. Phosphopeptides were enriched on TiO2 columns (C and D), and samples were analyzed by MA...
flag=container。务必使用注册linuxONE用的邮箱,填完之后邮箱下面会出现提示,直接点click here再去验证邮件即可。 点击container并登陆,就可以看到欢迎界面。 点击右上角的目录(加载速度巨慢,IBM的工程师说就是土豆服务器,连100人的并发都支持不了),然后就可以看到若干软件包。选择openmplbank并点击config,填个发行版...
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