Protein TagFLAG Sequencing PrimerForward:T7(TAATACGACTCACTATAGGG) Reverse:BGH(TAGAAGGCACAGTCGAGG) Schematic of pCMV3-C-FLAG-NCV (Negative Control Vector) Multiple Cloning Sites 最近浏览 pCMV3-C-FLAG Negative Control Vector (C-terminal FLAG-tagged)...
碧云天研发生产的His标签蛋白(C-Terminal)超快速Western检测试剂盒(Superquick Western Assay Kit for His Tag (C-terminal Specific)),是一种仅需10分钟就可以从完成转印的印迹膜到获得带His Tag (C-terminal Specific)标签的融合蛋白条带的超快速Western检测试剂盒。本试剂盒包含了C端特异性的Anti- His Tag-HRP...
碧云天研发生产的His标签蛋白(C-Terminal)超快速Western检测试剂盒(Superquick Western Assay Kit for His Tag (C-terminal Specific)),是一种仅需10分钟就可以从完成转印的印迹膜到获得带His Tag (C-terminal Specific)标签的融合蛋白条带的超快速Western检测试剂盒。本试剂盒包含了C端特异性的Anti- His Tag-HRP...
The encoded tags are FLAG, 3 × FLAG, T7, His-tag, Strep-tag II, S-tag, Myc, HSV, VSV-G and V5. The same pair of primers can be used to construct tagged alleles of a gene of interest with any of the 10 tags. In addition, a six-glycine linker sequence is inserted upstream...
APAF1, His-tag, FLAG-tag Recombinant 面议 BCL-2, His-Tag Recombinant 面议 BCL2A1, His-Tag Recombinant 面议 BCL2L10, His-Tag Recombinant 面议 Caspase6, His-tag Recombinant 面议 Caspase7, His-tag Recombinant 面议 产品简介 ●Purchase●DescriptionRecombinanthumanPCSK9(proproteinconvertasesubtil...
APP C-terminal fragment C83 with V5-tag on the C-terminus (C83-V5) was amplified by PCR using pCAGOSF-APP770 as template. Amplified Flag-C99, Flag-C83, and C83-V5 were subcloned into pQCXIP vector at NotI and PacI sites. The C99 site mutants K687A, K699A, K687,699A, K724,725...
To map the progerin/lamin A domain involved in BUBR1 binding, we created a series of truncations of progerin and lamin A28: the GST/Flag-tagged progerin N-terminal region (progerin-N; amino acid (aa) residues 1–300), the progerin middle region (progerin-M; aa residues 301–564) ...
For genome-wide CRISPR screening, K562 cells were made competent for inducible gene knockout (iCas9) by transduction with vectors SRPB (pHR-SFFV-rtTA3-PGK-Bsr) and 3GCasT (pHR-TRE3G-hSpCas9-NLS-FLAG-2A-Thy1.1). Cas9-P2A-Thy1.1 expression was induced with doxycycline for 2 days and ...
The FLAG-tagged c-FLIPS deletion mutant was constructed by PCR and cloned into the EcoRI and XhoI sites in-frame with the N-terminal FLAG tag in pCR3-Met-Flag. The HA-tagged ubiquitin was a kind gift from Dr. Dirk Bohmann (University of Rochester, Rochester, NY). Transient Transfection...
TNF Receptor Purification TNFRSC was purified from the indicated cell lines after stimulation with 100 ng/mL Flag-TNFα (Human TNFα, from Alexis) for 10 min. Ice-cold PBS was added to the plate to stop stimulation. Following lysis, TNFRSC was purified by incubation with Flag M2 agarose ...