根据实验结果,选取适当浓度的LPS、白藜芦醇及Sirtinol,将BV-2细胞分为对照组、LPS组、白藜芦醇+LPS组、Sirtinol+LPS组,分别用ELISA方法检测各组细胞相应处理12、24h后上清液中的IL-6和TNF-α的含量.Westemblotting检测SIRT1在上述各组细胞加药处理24h后的表达水平。结果与对照组相比,LPS组BV-2细胞数目增多,胞体肿胀...
IL一6对BY2小胶质细胞系铁代谢相关蛋白的影响及其调控机制研究摘要帕金森病( Parki nson’ S di sease,PD) 以静止性震颤、肌强直、运动迟缓、姿势反射障碍为主要症状。病理上表现为中脑黑质致密带( substanti a ni gra pars com pact,SN pc) 中多巴胺( dopam i ne,DA) 能神经元丢失,进而导致纹状体中DA...
(IL-1p)andinterleukin6(IL一6)levelsincreaseinthecerebrospinalfluidofPDpatients.i-methyl一4一phenyl一1,2 3,6一tetrahydropyridi九e(MPTP),6-hydroxydopamine(6-OHDA),rotenoneandannonacininducemicrogliaactivationinthesubstantianigra(SN)ofPDanimalmodels.WheninflammationoccurringinSNofthePDpatients,ironisusually...
目的 观察脂多糖(LPS)对BV-2细胞形状变化及白介素-6(IL-6)和肿瘤坏死因子-α(TNF-α)分泌情况的影响,探讨沉默信息调控因子1(SIRT1)在LPS诱导BV-2细胞释放前炎症因子过程中的作用. 方法 BV-2细胞分为调零组、对照组及LPS组,调零组不含细胞,只加培养基;对照组常规培养;LPS组分别加入不... 查看全部>> ...
目的观察枸橼酸铁铵(FAC)对BV2小胶质细胞白细胞介素1β和6(IL-1β和IL-6)mRNA表达的影响.方法 FAC组采用100μmol/L的FAC溶液刺激BV2细胞12h,对照组以DMEM培养液处理,应用荧光定量PCR技术检测两组IL-1β和IL-6mRNA表达量.结果 FAC组和对照组比较,IL-1βmRNA表达量差异无显著性(P〉0.05);而IL-6mRNA表达...
IL - 6也是炎症反应中的关键细胞因子。 实验表明天麻素能抑制IL - 6在脂多糖刺激下的升高。 炎症相关蛋白的表达变化能反映炎症机制。 Western blot技术用于检测iNOS蛋白的表达。 脂多糖诱导使iNOS蛋白表达明显上调。 天麻素处理后iNOS蛋白表达水平有所下降。 COX - 2蛋白在炎症过程中发挥重要作用。研究发现天麻素可...
Objective: To determine the effect of medicated serum of Chinese herbal compound Naofucong (脑复聪, NFC) on the microglia BV-2 cells viability and the transcription and expression of interleukin-6 (IL-6) and tumornecrosis factor α (TNF-α) in microglia BV-2 cells to further explore the ...
At a concentration of 80 μM, 8-epiasterolid significantly suppressed the LPS-induced production of IL-1β, IL-6, and TNF-α in both RAW264.7 and BV2 cells (Fig. 5). Fig. 5 The effect of 8-epiasterolid on the LPS-induced production of pro-inflammatory cytokines in RAW264.7 (A–...
However optical pure 1a presented significant inhibitory action on IL-6 and IL-1β mRNA expression at 10 μM, while 1b did not display inhibitory activity. Therefore, the SAR and stereo-selectivity of cycloneolignans on inhibitory effects against overactivation of BV2cells in vitro was ...
International Journal of Molecular Sciences Article Aspirin down Regulates Hepcidin by Inhibiting NF-κB and IL6/JAK2/STAT3 Pathways in BV-2 Microglial Cells Treated with Lipopolysaccharide Wan-Ying Li 1,2,†, Fei-Mi Li 1,†, Yu-Fu Zhou 1, Zhong-Min Wen 2,*, Juan Ma 1,3, Ke Ya ...