Primer sequences in qPCR. Additional file 4: Table S3. The results of Univariate cox regression analysis. Additional file 5. Table S4. The results of GSEA analysis between high- and low- risk groups. Additional file 6. Table S5. The results of subclass mapping for immunotherapy response. ...
The microarray data of IER3 in the GSE16011 set were downloaded from the Gene Expression Omnibus (GEO) database (https://www.ncbi.nlm.nih.gov/geo/). Gliomas with age < 18 and incomplete clinical and pathological follow-up information were excluded, such as age, gender, isocitrate ...
Single-cell profiling of tumor heterogeneity and the microenvironment in advanced non-small cell lung cancer - PubMed. https://pubmed.ncbi.nlm.nih.gov/33953163/. Kinker, G. S. et al. Pan-cancer single-cell RNA-seq identifies recurring programs of cellular heterogeneity. Nat. Genet. 52, 120...
4G–I). In another two RNA-seq datasets for advanced non-small cell lung cancer (NSCLC) patients who were treated with immunotherapy ± chemotherapy (GSE135222 and Orient-11), a high PPARG score was associated with a better prognosis (Fig. 4J–K). Additionally, the PPARG regulon was ...
Single-cell RNA sequence data from 6 samples were analyzed while studying the GSE188711 (including 19,872 CRC cells) dataset for CRC. The “Seurat” R package122was utilized to construct S4 objects, and then the “Harmony” R package123was used to integrate data from multiple samples. To en...
Despite its widespread use, RNA-seq is still too laborious and expensive to replace RT-qPCR as the default gene expression analysis method. We present a novel approach, BRB-seq, which uses early multiplexing to produce 3′ cDNA libraries for dozens of sa
RNA sequencing experiments generate large amounts of information about expression levels of genes. Although they are mainly used for quantifying expression levels, they contain much more biologically important information such as copy number variants (CN
mUC, metastatic urothelial cancer; CR, complete response; PR, partial response; SD, stable disease; PD, progressive disease; adNSCLC, advanced non-small cell lung cancer; PFS, progression-free survival; DCB, durable clinical benefit; NDB, no durable benefit; IC50, semi-inhibitory concentration. ...
(n = 32) and advanced (n = 32) lesions (left) and non-IPH (n = 16) and IPH (n = 27) lesions. non-IPH, non-intraplaque haemorrhage; IPH, intraplaque haemorrhage. ***,P ≤ 0.0001. Wilcoxon rank sum test.dHeatmap showing different blocks of DEGs along the...
Colorectal cancer is a malignant tumor of the digestive system originating from abnormal cell proliferation in the colon or rectum, often leading to gastro