The combination of breast cancer PDO and mini‐PDX platform for drug screening and individualized treatmentdoi:10.1111/jcmm.18374Cui, YuxinRan, RanDa, YanyanZhang, HuiwenJiang, MengQi, XinZhang, WeiNiu, LigangZhou, YuhuiZhou, CanJournal of Cellular & Molecular Medicine...
Abstract 4677: A preclinical platform of breast cancer PDX and derived cellular models as a tool for pharmacological screening and functional studies Despite considerable progress in understanding the biology and genetics of breast cancer, the development of effective therapies needs physiological and predic...
The ALDEFLUOR kit (StemCell Technologies, Durham, NC, USA) was also used to enrich the CSC population based on the high ALDH enzymatic activity35. Cells freshly dissociated from the breast cancer PDX TN-1 xenografts were suspended in ALDEFLUOR assay buffer containing ALDH substrate and incubated f...
Following acute treatment of the mice with lapatinib or vehicle for 48 h before harvesting, we observed a considerable decrease in the expression of ERRα in the lapatinib-treated NIC and PDX tumours (Fig. 2e,f). As observed in the cell line models, this decrease in ERRα levels was ...
Breast cancer Organoid Individualized treatment Abbreviations BC Breast cancer PDO Patient-derived organoid TME Tumor microenvironment PDX Patient derived xenograft ER Estrogen receptor PR Progesterone receptor HER2 Epidermal growth factor receptor 2 1. Introduction Currently, incidence rates of breast cancer ha...
This method is commonly used for drug screening, where drugs are added to the culture to evaluate their effectiveness in killing cancer cells. The viability of cells can be measured through various assays, including cell proliferation and apoptosis assays. (B) Patient-derived xenograft (PDX) ...
Therefore, we utilized breast cancer PDX from the Mayo Breast Cancer Genome Guided Therapy Study (BEAUTY) to establish two immortalized, genomically unique breast cancer cell lines. Through extensive genetic and therapeutic testing, the cell lines were found to retain the same clinical subtype, major...
To assign scRNA-seq transcriptomes to clones, we first generated CNA phylogenetic trees with a Bayesian [27] method from DLP+ single-cell genome sequencing of 6 patient PDX lines, to determine the genome fraction occupied by TNBC breast cancer clones. For each patient series, we identified CNA...
The heterogeneity of breast cancer plays a major role in drug response and resistance and has been extensively characterized at the genomic level. Here, a single-cell breast cancer mass cytometry (BCMC) panel is optimized to identify cell phenotypes and
Although these observations suggest a pan-breast cancer utility for proteasome inhibitors, our further experiments with breast cancer cell lines and patient-derived xenografts (PDX) pointed to triple-negative breast cancer (TNBC) as the most sensitive subtype to proteasome inhibition. Finally, using ...