Full size image In addition, many somatic mutations in the BRCA2 gene are observed among ER-positive BC. E2 promotes the rapid proliferation of cancer cells through the ER signaling pathway, along with the increase of DNA replication and damage of cancer cells (indirect mutagenesis effect) and ...
To gain insights into the mechanisms by which the SNVs disrupt BRCA2 activity, the location and influence of the PStrongmissense-induced changes on protein structure were evaluated. PStrongmissense variants were enriched in the helical domain and the OB1 domain (15.3% and 16.4%, respectively). ...
Author Contributions: Dr Antoniou had full access to all of the data in the study and takes responsibility for the integrity of the data and the accuracy of the data analysis. Drs Hopper, Kuchenbaecker, and Barnes are joint first authors. Drs Rookus, Easton, and Antoniou are joint senior ...
Full size table In the BROCADE series of trials, veliparib was combined with platinum-based chemotherapy. The phase II BROCADE trial [41,42,43,44] revealed a numerical but not significant difference in PFS and overall survival (OS) between the veliparib and control arms. However, there was a...
in breast cancer Pingping Gao, Cancer Innovation, 2022 A lactate‐responsive gene signature predicts the prognosis and immunotherapeutic response of patients with triple‐negative breast cancer Kaixiang Feng, Cancer Innovation, 2024 Advances in medical treatment of breast cancer in 2022 Jingtong Zhai, ...
2.11. In vitro pull-down assay The expression of Myc-BAP1 was conducted referring to the previous protocol30. Briefly, the full length of BAP1 was cloned into pcDNA3.1 and transfected into HEK293F. Myc-BAP1 was purified with anti-Myc magnetic beads from cell lysates. Biotin-labeled PT33 ...
A detailed investigation using long-term mammary organoid cultures revealed that healthy mammary glands are not predisposed to form lesions that precede tumors. However, when exposed to stress during DNA replication, the population of hormone receptor-negative (HR-) luminal cells significantly expanded....
In addition to the overall weaker staining, BRCA2 focus formation was also largely abrogated by PALB2 depletion. Furthermore, no distinct BRCA2 foci were observed even after IR in PALB2-depleted cells (Figure S2B). Download: Download high-res image (1MB) Download: Download full-size image ...
Supplementary Figs S8–S15 show full gel images of western blots and of the one Southern blot shown in other figures. Cell lines and cell culture. The Brca1fl/mut ES cell line (11CO/47T) was a kind gift from Dr Alan Ashworth37. ES cells were grown in ES medium on either MEF ...
© American College of Medical Genetics and Genomics Original Research Article Cigarette smoking in women after BRCA1/2 genetic test disclosure: a 5-year follow-up study of the GENEPSO PS cohort Claire Julian-Reynier, MD, MSc1–3, Noémie Resseguier, MD, PhD2,3, Anne-Deborah...