For sequencing LPS-TRAPed cells, we used TRAP2;Ai9 mice that were TRAP-labelled with tdTomato in response to LPS. Cells from the cNST were isolated as described above. The cell suspension was stained with DRAQ5 (62254, Thermo Scientific) and Calcein Violet (C34858, Thermo Scientific) to ...
Over 170 years ago, the accident of American railroad worker Phineas Gage first revealed that specific brain regions were important for certain functions. Since then, various research work has been performed to understand the brain regions, brain cells and their functions, while the rapid progress...
A major drawback of these studies is that they employ transgenic mice for each cell type with genetically labeled cells for isolation and sorting. Moreover, some of the above studies are based on single-cell sorting, which is tedious, expensive and not easily applicable to disease states. ...
The spatial average of those averages is the plot labeled “Average”. Channel FC33 was excluded due to its high impedance value for this participant. The vertical broken line represents the movement onset (MO). Table 4. Hyperparameter optimization: closed-loop model hyperparameter optimization...
This data-driven PFC column model in BrainCog provides an effective simulation-validation platform to study other high-level cognitive functions.66 Download: Download high-res image (638KB) Download: Download full-size image Figure 8. Anatomical and network simulation diagram (A) The connection of...
Build the Paper Circuit with Copper TapeNow it’s time to build the circuit!Open card to circuit diagram side. Copper tape from the negative LED lead along the negative circuit line to the break in the circuit line. Copper tape the rectangle on the instructions side labeled copper tape. ...
Servicebio). Membranes were washed three times with Tris-buffered saline with 0.05% Tween-20 and incubated for 1 h at room temperature with horseradish peroxidase-labeled anti-rabbit, anti-goat, or anti-rat secondary antibody (1:5000, Invitrogen). Target proteins were visualized with enhanced chem...
A simplified top view diagram of the LSFM showing its main components and relative optical setup. The close up highlights the approximate rectangular profile of a Gaussian sheet intersection with the specimen. Full size image A physically-plausible simulation of the LSFM requires accurate modelling of...
The expression in all 3 sites reflects the expression of the native brv1 gene as all are labeled in in situ hybridization experiments with an antisense brv1 probe (Figure S2). Could any of these neurons be the elusive antennal cold receptors? To answer this question, we expressed G-CaMP, ...
6). Importantly, multiple independently-imaged groups of untreated siblings (labeled WT-0h on Fig. 2c) cluster with one another, as do multiple independently-imaged groups of untreated mutants (Mut-0h on Fig. 2c), verifying that functional connectivity patterns are relatively uniform for a ...