质谱技术是蛋白质组学(proteomics)研究中的关键技术。在蛋白质质谱领域,主要有两种分析策略:Top-down(自上而下)和Bottom-up(自下而上)。这两种方法在处理和分析蛋白质样品时有本质上的不同。 1、Top-down策略(自上而下): 在Top-down方法中,整个蛋白质直接被引入到质谱仪中,并在质谱仪内部被解离为较小的片段...
质谱技术是蛋白质组学(proteomics)研究中的关键技术.在蛋白质质谱领域,主要有两种分析策略:Top-down(自上而下)和Bottom-up(自下而上).这两种方法在处理和分析蛋白质样品时有本质上的不同.
top-down是常说的intact analy…质谱技术是蛋白质组学（proteomics）研究中的关键技术。在蛋白质质谱领域...
Liquid chromatography–tandem mass spectrometry (LC–MS/MS)-based proteomics is a powerful technique for profiling proteomes of cells, tissues, and body fluids. Typical bottom-up proteomic workflows consist of the following three major steps: sample preparation, LC–MS/MS analysis, and data ...
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Bottom-Up Proteomics 来自 Ingenta 喜欢 0 阅读量: 72 作者: A Armirotti 摘要: In this work, the bottom-up protein identification process is described, from sample digestion to the final database search result. Both MALDI and LC-MS approaches to the identification issue are illustrated. The ...
Bottom-Up Proteomics. 来自 EBSCO 喜欢 0 阅读量: 26 作者:Armirotti,Andrea 摘要: In this work, the "bottom-up" protein identification process is described, from sample digestion to the final database search result. Both MALDI and LC-MS approaches to the identification issue are illustrated. ...
Apolipoprotein L1mass spectrometryliquid chromatographybottom-up proteomicsdry plasmamicrosamplegenotypeA targeted, bottom-up proteomic assay was developed for the qualitative detection of apolipoprotein L1 (ApoL1) protein variants (G0, G1, and G2) in blood plasma for identification of high and low ...
Bottom-Up Proteomics 作者:Frederic P·Miller/Agnes F·Vandome/John McBrewster 页数:68 ISBN:9786132593832 豆瓣评分 目前无人评价 我要写书评 Bottom-Up Proteomics的书评 ···(全部 0 条)
Protein analysis by shotgun/bottom-up proteomics. Bottom-up protein analysis refers to the characterization of proteins by analysis of peptides released from the protein through proteolysis. When bottom-up... Y Zhang,BR Fonslow,B Shan,... - 《Chemical Reviews》...