质谱技术是蛋白质组学(proteomics)研究中的关键技术.在蛋白质质谱领域,主要有两种分析策略:Top-down(自上而下)和Bottom-up(自下而上).这两种方法在处理和分析蛋白质样品时有本质上的不同.
质谱技术是蛋白质组学(proteomics)研究中的关键技术。在蛋白质质谱领域,主要有两种分析策略:Top-down(自上而下)和Bottom-up(自下而上)。这两种方法在处理和分析蛋白质样品时有本质上的不同。 1、Top-down策略(自上而下): 在Top-down方法中,整个蛋白质直接被引入到质谱仪中,并在质谱仪内部被解离为较小的片段...
Bottom-up策略首先使用特定的酶（通常是胰蛋白酶）对蛋白质进行消化，产生肽段。
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Bottom-Up Proteomics 来自 Ingenta 喜欢 0 阅读量: 72 作者: A Armirotti 摘要: In this work, the bottom-up protein identification process is described, from sample digestion to the final database search result. Both MALDI and LC-MS approaches to the identification issue are illustrated. The ...
Liquid chromatography–tandem mass spectrometry (LC–MS/MS)-based proteomics is a powerful technique for profiling proteomes of cells, tissues, and body fluids. Typical bottom-up proteomic workflows consist of the following three major steps: sample preparation, LC–MS/MS analysis, and data ...
Bottom-Up Proteomics 作者:Frederic P·Miller/Agnes F·Vandome/John McBrewster 页数:68 ISBN:9786132593832 豆瓣评分 目前无人评价 我要写书评 Bottom-Up Proteomics的书评 ···(全部 0 条)
Bottom-Up Proteomics. 来自 EBSCO 喜欢 0 阅读量: 26 作者:Armirotti,Andrea 摘要: In this work, the "bottom-up" protein identification process is described, from sample digestion to the final database search result. Both MALDI and LC-MS approaches to the identification issue are illustrated. ...
bottom-up proteomicsdry plasmamicrosamplegenotypeA targeted, bottom-up proteomic assay was developed for the qualitative detection of apolipoprotein L1 (ApoL1) protein variants (G0, G1, and G2) in blood plasma for identification of high and low renal risk genotypes. Following trypsin digestion of...
摘要: In this chapter we provide an overview of bottom-up proteomic approaches. These allow the identification and characterization of proteins and their amino acid sequences, including post-translational...关键词:Liquid chromatography Mass spectrometry Heart Proteomics MudPIT ...