Genomic DNA was extracted from frozen tissue using the Gentra Puregene isolation kit. We analyzed tumor genome-wide copy number alterations using array comparative genomic hybridization (array CGH). Agilent 244k oligonucleotide arrays were hybridized with tumor DNA labeled in CY5 and control normal DNA...
Osteoblasts are clearly seen active in the tissue surrounding the scaffold struts, with clear osteocytes embedded in mineralized matrix in the scaffold pores (labeled with white arrowheads). We demonstrate that this rationale, as depicted schematically in Fig. 2 leads to structural and functional ...
Gli1-labeled adult mesenchymal stem/progenitor cells and hedgehog signaling contribute to endochondral heterotopic ossification. Bone 109, 71–79 (2018). CAS PubMed Google Scholar Eisner, C. et al. Murine tissue-resident PDGFRα+ fibro-adipogenic progenitors spontaneously acquire osteogenic phenotype ...
bone tissue engineering; 3D magnetic scaffolds; biomaterials; magnetic nanoparticles1. Introduction In recent years, the bone disorders and diseases has drastically increased worldwide, and this tendency is expected to continue in the near future, due to the risks related to aging of the population,...
We examined SOST gene expression in a set of 76 human tissues and cell lines by probing the Multiple Tissue Expression Array (Clontech) with a fragment corresponding to the second exon; aorta and fetal kidney exhibited the highest levels of expression (not shown). It is noteworthy, however, ...
Interest in obtaining bioactive glasses in the form of nanoparticles has been increasing in the recent decades due to their potential applications in bone tissue regeneration, soft tissue applications, and wound healing. Moreover, the development of sol–gel chemistry has provided new possibilities for...
infection of the soft tissue and bone, and breakage of the fillers with or without particulate formation. Considering the adverse reaction, surgeons often prefer established techniques due to a proven track record, which leads to the limited utilization of bone void fillers, hampering market growth...
Immunohistochemistry (IHC) is a well-established, widely accepted method in both clinical and experimental parts of medical science. It allows receiving valuable information about any process in any tissue, and especially in bone. Each year the amount of
Subsequently, these sections were incubated with secondary antibodies labeled with horseradish peroxidase. Diaminobenzidine was added to these tissues for color development. The tissue sections were then counterstained with hematoxylin, dehydrated, incubated with xylene to make them transparent. All tissue ...
To produce effective treatment of bone tumours, it is essential to eradicate all tumour tissue. Extracorporeal devitalisation and reimplantation requires both a safe margin of resection and also definite eradication of all tumour cells. In 1928, Friedgood [6] was the first to show that Walker ...