Bone defects, resulting from substantial bone loss that exceeds the natural self-healing capacity, pose significant challenges to current therapeutic approaches due to various limitations. In the quest for alternative therapeutic strategies, bone tissue
Systematic evaluations were conducted at 10 days post-implantation using histology, histomorphology, immunohistochemistry, X-ray microscopy and energy dispersive X-ray spectroscopy (EDX) analysis to assess mechanical sensing protein PIEZO1 expression and related healing process of peri-implant bone. This ...
Osteocytes were fixed in 4% paraformaldehyde and stained with anti-podoplanin antibody (8.1.1; sc-53533, Santa Cruz Biotechnologies, 1:50), an osteocyte marker, followed by staining of Alexa Fluor 488-labeled goat anti-hamster IgG (H + L) Cross-Adsorbed Secondary Antibody (A-21110, The...
The comprehensive techniques presented here, which include microcomputed tomography, advanced mineralized hard-tissue resin histology, scanning electron microscopy and small angle x-ray scattering provides key insight into the cellular and extracellular matrix function and organization pertaining to long-term ...
In some instances, emGFP-Slc37a2 expressing cells were labeled with LysoTrackerTM Red DND-99 (Thermo Fisher Scientific, 1:1000 dilution) probes or co-transfected with late-endosomal/lysosomal markers (mRFP-Rab7 or LAMP1-RFP) and imaged live using the NIKON A1R in a Ti-E inverted motorized ...
Histology Figure 2 shows cross-section microphotographs of the bone tissue inside the callus with Villanueva Osteochrome Bone Table 3 Bone histomorphometric analysis of osteotomy site at the femoral diaphysis Vehicle (n = 8) Celecoxib (n = 8) Vitamin K2 (n = 5) Total Ar (mm) Callus Ar ...
Additionally, “Weakly supervised machine learning” offers techniques to leverage imperfect data, enhancing our model’s robustness when high-quality labeled data is scarce [31]. Lastly, “Deep learning in food category recognition” highlights the benefits of information fusion from multiple data ...
E: Histology of bone lesions induced by C4-2B/luc+ cells. H&E-stained section of the proximal left tibia 90 days after the intraosseous inoculation of C4-2B/luc+ cells. b, bone trabeculae; c, cancer cells. Arrowheads indicate bone-forming surfaces covered by active osteoblasts. Synthesis ...
hyperspectral imaging, and mass spectrometry imaging, enable the visualization and analysis of the spatial distribution of biomolecules, cells, and tissues. Linking these molecular imaging outputs to conventional tissue histology can facilitate a more comprehensive characterization of disease phenotypes. This ...
Recent interest in the control of bone metabolism has focused on a specialized subset of CD31hiendomucinhi vessels, which are reported to couple angiogenesis with osteogenesis. However, the underlying mechanisms that link these processes together remain