Label-free and slide-free histology via ultraviolet photoacoustic microscopy may allow for rapid diagnoses of bone-tissue pathologies and aid the intraoperative determination of tumour margins. This is a preview of subscription content, access via your institution Access options Access Nature and 54 ...
Design: For all 34 cases, histology slides were reviewed by two pathologists, one of whom is a subspecialized bone and soft tissue pathologist. The following pathological features were noted: tumor location, tumor size, pathological subtype, tumor grade, margin status, response to neoadjuvant ...
We therefore evaluated pulp mineralization induced by transplantation of a green fluorescent protein (GFP)-labeled tooth into a GFP-negative hypodermis of host rats. Five days after the transplantation, the upper pulp cavity became necrotic; however, cell-rich hard tissue was observed adjacent to ...
Bone defects, resulting from substantial bone loss that exceeds the natural self-healing capacity, pose significant challenges to current therapeutic approaches due to various limitations. In the quest for alternative therapeutic strategies, bone tissue
The comprehensive techniques presented here, which include microcomputed tomography, advanced mineralized hard-tissue resin histology, scanning electron microscopy and small angle x-ray scattering provides key insight into the cellular and extracellular matrix function and organization pertaining to long-term ...
Immunohistochemistry (IHC) is a well-established, widely accepted method in both clinical and experimental parts of medical science. It allows receiving valuable information about any process in any tissue, and especially in bone. Each year the amount of
For the histology of decalcified bone tissue, HE staining by paraffin section further confirmed the protection of PL, PL-exo and PL-exo-ALN on MPS induced bone loss, which was in keeping with the above undecalcified histology and micro CT analysis (Fig.7A). By magnifying the HE images, ...
Especially, there are two essential steps to accelerate bone tissue regeneration: one is the early-staged recruitment of stem cells to injured sites, another is the further osteogenic differentiation of migrated cells and the mineralization of extracellular matrix [7]. Considering the therapeutic ...
Genomic DNA was extracted from frozen tissue using the Gentra Puregene isolation kit. We analyzed tumor genome-wide copy number alterations using array comparative genomic hybridization (array CGH). Agilent 244k oligonucleotide arrays were hybridized with tumor DNA labeled in CY5 and control normal DNA...
Design: 31 tissue microarrays (TMAs) containing benign and malignant neoplasms, reactive lesions and control tissue were labeled with Sox10 IHC. Nuclear labeling >1% was considered positive. TMAs contained 518 non-neuroendocrine carcinomas (adrenal, bladder, endometrial, gastric, esophageal, liver, ...