We also performed DNA sequencing on all coding exons of the IDS gene in formalin-fixed paraffin-embedded lymphoid tissue from each patient. Results: DNA sequencing of the IDS gene revealed different pathogenic mutations in each patient: one novel single-base deletion in exon 6 creating an in-...
Bone & Soft Tissue 34 Novel Kinase Mutations in KDR/VEGFR2, TIE1, and SNRK in Angiosarcoma (AS) Patients CR Antonescu, A Yoshida, NP Agaram, NE Chang, RG Maki. Memorial Sloan-Kettering Cancer Ctr, New York, NY. Background: The pathogenesis of AS is not well understood and anecdotal ...
Osteoblasts are clearly seen active in the tissue surrounding the scaffold struts, with clear osteocytes embedded in mineralized matrix in the scaffold pores (labeled with white arrowheads). We demonstrate that this rationale, as depicted schematically in Fig. 2 leads to structural and functional ...
Sign in to download full-size image Figure 8.22. Subdermal connective tissue sheath (SDS) of the bottlenose dolphin, Tursiops truncatus (blubber and m. cutaneous trunci removed).From Pabst (1990). The vertebral column is the primary structure used by dolphins to generate the dorsoventral bending...
Immunohistochemistry (IHC) is a well-established, widely accepted method in both clinical and experimental parts of medical science. It allows receiving valuable information about any process in any tissue, and especially in bone. Each year the amount of
using the gaussian point-spread functions. the oscgm-based xq and xb are equipped with depth-dependent 3d resolution recovery using actual measured point-spread functions map over the entire fov. the xb algorithm divides ct pixels into six tissue classes with smooth boundaries based on ct values...
Subsequently, these sections were incubated with secondary antibodies labeled with horseradish peroxidase. Diaminobenzidine was added to these tissues for color development. The tissue sections were then counterstained with hematoxylin, dehydrated, incubated with xylene to make them transparent. All tissue ...
Histology of sterilized specimen showing necrotic tumour osteoid( ). No viable tumour cells. Full size imageDiscussion To produce effective treatment of bone tumours, it is essential to eradicate all tumour tissue. Extracorporeal devitalisation and reimplantation requires both a safe margin of resection...
Osteomyelitis is a devastating disease caused by microbial infection in deep bone tissue. Its high recurrence rate and impaired restoration of bone deficiencies are major challenges in treatment. Microbes have evolved numerous mechanisms to effectively e
After removing the periosteum and bone marrow, the remaining bones were physically crashed to small pieces using a tissue homogenizer and then subjected to digestion by collagenase II (; Gibco) for 3 h at 37 °C. The digested tissues were filtered through 70-µm nylon mesh membranes (...