Methods: We collected publicly available single cell transcriptomic data of 145 healthy samples encompassing a wide spectrum of ages ranging from 2 to 84 years old to construct our human bone marrow atlas. The final atlas has 673,750 cells and 54 annotated cell types. ...
a childhood tumor has been molecularly defined at the primary cancer site, however, the bone marrow (BM) as the metastatic niche of NB is poorly characterized. Here we perform single-cell transcriptomic and epigenomic profiling of BM aspirates from 11 subjects spanning three major NB subtypes and...
2 A single-cell atlas of human FBM. 2a) Continuous decision tree constructed using the Rpart package to distinguish between the lineage-committed immune cell types in FBM (total) CITE-seq data using the 198 epitopes detected (see Supplementary Methods). Node splits were labelled ‘lo’ or ...
Single-cell analysis of the fate of c-kit-positive bone marrow cellsAnna Czarna, Fumihiro Sanada, Alex Matsuda, Junghyun Kim, Sergio Signore, João D. Pereira, Andrea Sorrentino, Ramaswamy Kannappan, Antonio Cannatà, Toru Hosoda, Marcello Rota, Filippo Crea, Piero Anversa...
P2RX4 promotes plasma cell survival by regulating endoplasmic reticulum homeostasis, as short-term P2RX4 blockade caused accumulation of endoplasmic reticulum stress-associated regulatory proteins including ATF4 and B-lineage mutation of the pro-apoptotic ATF4 target Chop prevented bone marrow plasma cell ...
To achieve this, we conducted a comprehensive single-cell analysis of bone marrow cells from both the bone and the heart to characterize the entire population of bone marrow-derived cells that home to the heart under both homeostatic and ischemic conditions. Methods Rats Animal procedures were ...
bone marrow cellcultures, they demonstrated that stromal cells quickly adhered to the culture flask and were easily separated from hematopoietic cells (nonadherent fraction). Under proper conditions, some of these cells formed colonies of fibroblastoid cells, each colony derived from a singlecell ...
Figure 1. A single-cell transcriptomic atlas of hematopoietic and non-hematopoietic cells of human bone marrow (A) Schematic for the scRNA-seq workflow. Magnetic-activated cell sorting (MACS) separation of hematopoietic, stem/progenitor, and mesenchymal fractions was performed and then pooled into ...
However, since neutrophils are very RNA-poor, single-cell profiling of these cells has been technically challenging. Here, we generated a single-cell RNA-seq dataset of primary neutrophils from adult female and male mouse bone marrow. After stringent quality control, we found that previously ...
Single-cell RNA sequencing analysis reveals that a unique atlas of in situ stem/progenitor cells is generated during the calvarial bone healing in vivo. Notably, we find a local expansion of resident Msx1+ skeletal stem cells after transplantation of the in situ cell culture system. Moreover, ...