The present study investigates crucial roles of BMP11 in cultured 3T3-L1 white and HIB1B brown adipocytes, using Bmp11 gene depletion and pharmacological inhibition of BMP11. The silencing of Bmp11 markedly decreases the expression levels of brown-fat signature proteins and beige-specific genes in...
在此基础上克隆了泰山赤鳞鱼BMP11的cDNA序列,并研究了此基因在不同胚胎发育时期及不同组织中的表达状况。从泰山赤鳞鱼的cDNA中扩增出一条643bp的条带,与斑马鱼的BMP11序列同源性高达92.34%,证明了此基因在不同物种间有高度的保守性。选择了11个不同的胚胎发育时期研究BMP11基因的表达规律,结果表明:BMP11基因在...
描述:Recombinant Human Growth differentiation factor 11 is produced by our Mammalian expression system and the target gene encoding Asn299-Ser407 is expressed. Accession:O95390 中文别名:GDF-11蛋白; BMP-11蛋白 来源:Human Cells 纯度:Greater than 95% as determined by reducing SDS-PAGE. ...
英文别名: Growth/differentiation factor 11,GDF-11,Bone morphogenetic protein 11,BMP-11 描述: Recombinant Human Growth differentiation factor 11 is produced by our Mammalian expression system and the target gene encoding Asn299-Ser407 is expressed. Accession: O95390 中文别名: GDF-11蛋白; BMP-11蛋...
HIF-1a and HIF-2a are closely related and activate HRE-dependent gene expression, sharing several common targets, such as VEGF, but also regulating distinct transcriptional targets that may require unique transcriptional cofactors. (16) HIF-1a is expressed ubiquitously, whereas HIF-2a expression is ...
(BMP) signaling in the context of an inflammatory milieu. (6–12) Soft tissue injury can lead to increased expression of BMP4 (13) and other osteogenic cytokines, (11) as well as to induction of the BMP intracellular effectors, Smads 1 and 5,(14) providing a potential pathophysiological ...
（图1）图1. BMP信号通路2.BMP报告基因检测（BRE-Luc Reporter Gene Assay）的原理和应用2.1检测原理 Id1启动子序列是BMP信号通路的下游响应元件。报告基因质粒是将Smad结合元件Id1启动子序列装在荧光素酶启动子位置。一旦BMP-Smad通路被激活，报告基因就会表达荧光素酶，通过检测荧光素酶的水平，可以高灵敏度...
A DNA sequence encoding part of the human BMP-11 protein (SEQ ID NO: 3) and the corresponding amino acid sequence (SEQ ID NO: 4) are set forth in the Sequence Listing. These sequences may also be used in order to design probes to obtain the complete human BMP-11 gene through ...
/BMP-2 microcarriers, and the highest level of Runx2 expression were found in the microcarrier treated with a medium concentration of BMP-2. Furthermore, slightly higher OPN gene expression at 7 days on PLGA/HA/BMP-2, GO-PLGA/HA/BMP-2 (50 ng mL−1), and GO-PLGA/HA/BMP-2 (100 ...
and translocate into the nucleus to regulate gene expression8,9,10,11. BMP receptors can also signal through non-Smad pathways such as p38, JNK and PI3K to regulate multiple cellular functions12,13. In intestine, BMP2 and BMP4 ligands are secreted by intravillus and intercrypt mesenchymal cel...