TaKaRa EpiTaq HS (for bisulfite-treated DNA) 说明书
Prevention of PCR cross-contamination by UNG treatment of bisulfite- treated DNA. Methods Mol Biol 507, 357-370 (2009).Tetzner R. Prevention of PCR cross-contamina- tion by UNG treatment of bisulfite-treated DNA. Methods Mol Biol 2009; 507: 357-370....
PCR amplification was conducted using FastStart High Fidelity DNA polymerase (Roche, IN, USA), for 42 cycles. DNA methylatio...Aranyi T, Tusnady GE. BiSearch: ePCR tool for native or bisulfite- treated genomic template. Methods Mol Biol 2007; 402:385-402; PMID:17951807; https://doi....
Methylation analysis of the H19-DMR, using bisulfite-treated genomic DNA.Tomoko FukeSeiji MizunoToshiro NagaiTomonobu HasegawaReiko HorikawaYoko MiyoshiKoji MuroyaTatsuro KondohChikahiko NumakuraSeiji Sato
The lambda phage DNA is free of DNA methylation, so all cytosine of the genome should have been converted to uracil. The unconverted cytosines thus reveal the unconversionr rate. Usage: $ ./bs3-unconversion -h Usage: ./bs3-unconversion -h [options] -f INPUT The path to the raw bis...
The lambda phage DNA is free of DNA methylation, so all cytosine of the genome should have been converted to uracil. The unconverted cytosines thus reveal the unconversionr rate. Usage: $ ./bs3-unconversion -h Usage: ./bs3-unconversion -h [options] -f INPUT The path to the raw bis...
comprising bisulfite-protected cytosines; (b) treating the ligated DNA molecules with bisulfite; (c) hybridizing the bisulfite-treated DNA molecules with a primer; (d) extending the hybridized primer to make double stranded DNA; and (e) in vitro transcribing the double-stranded DNA to make RNA...
Using DNA bisulfite treatment, reducible Cs are transformed into nonreducible uracil residues, strongly decreasing square wave voltammetric C reduction peaks. On the other hand, 5-methylcytosine (mC) residues resist the bisulfite treatment and display almost unchanged reduction peak. Desulfonation step ...
Prevention of PCR cross-contamination by UNG treatment of bisulfite- treated DNA. Methods Mol Biol 507, 357-370 (2009).Tetzner R. Prevention of PCR cross-contamina- tion by UNG treatment of bisulfite-treated DNA. Methods Mol Biol 2009; 507: 357-370....
comprising bisulfite-protected cytosines; (b) treating the ligated DNA molecules with bisulfite; (c) hybridizing the bisulfite-treated DNA molecules with a primer; (d) extending the hybridized primer to make double stranded DNA; and (e) in vitro transcribing the double-stranded DNA to make RNA...