TaKaRa EpiTaq HS (for bisulfite-treated DNA) 说明书
Tetzner R. Prevention of PCR cross-contamina- tion by UNG treatment of bisulfite-treated DNA. Methods Mol Biol 2009; 507: 357-370.Tetzner R (2009) Prevention of PCR cross-contamination by UNG treatment of bisulfite-treated DNA. Methods Mol Biol 507:357–370...
In the LABS protocol, a fully methylated T7 adaptor is ligated to each end of the DNA fragment, and the resulting construct is treated with bisulfite. The T7 adaptor includes a T7 RNA polymerase promoter sequence and a 3′-end blocked short helper to form a partial double-stranded DNA st...
and the excitation light can be captured to read the base information. Paired-end 150 bp strategy is typically employed in WGBS to sequence 250-300 bp insertion bisulfite-treated DNA libraries. In addition to Illumina HiSeq,PacBio SMRT,Nanopore, Roche 454, and other Illumina platforms are also...
Input starting material must be bisulfite-treated DNA generated from various input DNA amounts of 1 ng to 1µg. For optimal preparation, the input DNA amount for the bisulfite conversion process should be 100 ng to 200 ng so that sufficient bisulfite-treated DNA can be yielded. ...
DNA Methylation Analysis InDiagnostic Pathology: Molecular Oncology, 2016 Bisulfite Conversion Methods • Most common method of measuring methylation status • Genomic DNA is isolated and treated with sodiumbisulfite • Bisulfite causes chemical conversion of unmethylated cytosines to uracil ...
By comparing the sequence of the bisulfite-treated DNA with that of untreated DNA, the methylation profile is determined: conversion of a C to T indicates non-methylated C's; in contrast, the absence of C to T conversion indicates protection by the methyl moiety of the C and hence ...
The sequencing primers which will be added to the DNA fragments to process sequencing also can be treated as random amplication primer for DNA samples. Thus, primer designing is not necessary and work of PCR will be decreased when compared to Sanger sequencing to profile genome-wide DNA ...
One μg of genomic DNA was bisulfite treated using the Epimark Bisulfite Conversion Kit, and 2 μl of eluted DNA was analyzed by end-point PCR. 388, 544, and 731 bp amplicons were amplified with primer pairs for bisulfite converted DNA (lanes 1, 3, and 5), or with primer pairs for...
DNA methylation is an essential epigenetic modification in the human genome. For the investigation of DNA methylation patterns, bisulfite conversion and DN... Y Zhang,C Rohde,S Tierling,... - Humana Press 被引量: 70发表: 2009年 Fast and sensitive mapping of bisulfite-treated sequencing data ...