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Blood-based DNA methylation has shown great promise as a biomarker in a wide variety of diseases. Studies of DNA methylation in blood often utilize samples which have been cryopreserved for years or even decades. Therefore, changes in DNA methylation ass
In mammals, global passive demethylation contributes to epigenetic reprogramming during early embryonic development. At this stage, the majority of DNA-methyltransferase 1 (DNMT1) protein is excluded from nucleus, which is considered the primary cause. H
To date, most studies on insect DNA methylation are based primarily on the insect methylome sequencing and the manipulation of functionalDnmtgenes. By means of whole-genome bisulfite sequencing (WGBS-seq), a single-base resolution of methylome has so far been acquired in several insect species, i...
Cellular fractionation and western blotting revealed that BCL11A is found in the nucleoplasm and chromatin, and degraded by 2 h with drug (Figure 1D). Treatment over a range of dTAG-47 concentrations elicited the hook effect, which is characteristic of PROTAC molecules (Moreau et al., 2020) ...
DNA methylation is a crucial epigenetic mark for activity-dependent gene expression in neurons. Very little is known about how synaptic signals impact promoter methylation in neuronal nuclei. In this study we show that protein levels of the principal de
Bacterial degradation of organosulfonates plays an important role in sulfur recycling, and has been extensively studied. However, this process in anaerobic bacteria especially gut bacteria is little known despite of its potential significant impact on hu
Targeted protein degradation (TPD) relies on small molecules to recruit proteins to E3 ligases to induce their ubiquitylation and degradation by the proteasome. Only a few of the approximately 600 human E3 ligases are currently amenable to this strategy.
of organic pollutant treatment due to its high redox potential, long half-life and wide application range [14,15,16]. The key to PMS-based oxidation is the formation of highly reactive species. The general methods for activating PMS include the use of external energy (such as heat, ...
DNA Extraction and Bisulfite Pyrosequencing Genomic DNA was extracted using an AllPrep RNA/DNA Mini kit (Qiagen, Dusseldorf, Germany) following manufacturer's instructions. Bisulfite transformation and purification of 1-2 µg DNA were performed with an EpiTect Bisulfite Kit (Qiagen) according to ...