PacBio测序的原理是基于DNA聚合酶的活性。在测序过程中,DNA模板被固定在一个单个的微小孔中,该孔被称为SMRT细胞。然后,DNA聚合酶从DNA模板的单链上开始合成新的DNA链。DNA聚合酶在添加新的核苷酸时会释放出一个荧光信号,这个信号会被检测器记录下来。 PacBio测序的工作流程包括样本准备、测序反应、数据分析和结果解...
PacBiothird-generation sequencingThere is no consensus barcoding region for determination of arbuscular mycorrhizal fungal (AMF) taxa. To overcome this obstacle, we have developed an approach to sequence an AMF marker within the ribosome銆唍coding operon (rDNA) that covers all three widely applied ...
PacBio sequencing overcomes the high-sequence homology and hyper polymorphisms to identify true HLA allelic diversity.Access the full spectrum of polymorphisms across the HLA system Highly accurate long reads — HiFi reads — with single-molecule resolution make Single Molecule, Real-Time (SMRT) ...
5mC/5hmC Sequencing oxBS-seq hMeDIP-seq Nanopore RNA Methylation Sequencing Service RNA Methylation Sequencing Service 2'-O-RNA Methylation Sequencing Service PacBio SMRT Sequencing Long-Read Metagenomic Sequencing Bacterial Whole Genome de novo Sequencing Fungal Whole Genome de novo Sequencing Full-Le...
Iso-seq, developed by PacBio, is based on long-read sequencing technology and allows researchers to identify new isoforms with extraordinary precision.
Pacbio Sequencing Pacific Biosciences: Realtime Single Molecule Sequencing The PacBio Sequel uses proprietary SMRT (Single Molecule Real Time) technology, which enables the real-ti... 查看原文 二三代基因组混合组装流程的搭建与香菇基因组精细图谱的获得 ...
Sequencing systems Revio long-read system Vega long-read system Onso short-read system A groundbreaking new sequencer The Onso system is an innovative benchtop short-read DNA sequencing platform with an extraordinary level of accuracy using PacBio sequencing by binding (SBB) technology. Ultra-high ...
We develop a low-input (100 ng), low-cost, and amplification-free library-generation method for PacBio sequencing (LILAP) using Tn5-based tagmentation and DNA circularization within one tube. We test LILAP with two Drosophila melanogaster individuals, and generate near-complete genomes, ...
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