Biotechnology Curriculum Leads Students to Gene SequencingAt Sacred Heart Academy, Faith comes first as students and teachers keep an open dialogue about...Wuerth, Allyson
sequencing. When coupled with next-generation sequencing, our classification method should provide a more general and complementary approach to comparisons against known GMO-associated sequences because it can identify uncatalogued synthetic transgenes, such as those not intended for crop enhancement. In ...
For instances where the genome of a given organism is not well represented in the public databases, an important feature of the development of a training set is the presence of a correlated EST or cDNA based sequencing project. In cases where a full-length cDNA has been sequenced, the entire...
Whole genome sequencing to evaluate off-target effects of CRISPR-Cas9 editing In order to establish if the CRISPR-Cas9 gene editing caused any off-target effects that may have impacted on the mutant strains ability to produce enduracidin, six strains were subjected to PacBio whole genome sequenci...
Traditionally, quantitative PCR has been used to measure the co-immunoprecipitation of specific target sequences with a transcription factor of interest. However, when combined with high-throughput sequencing techniques (ChIP-Seq) it is now possible to obtain an unbiased view of transcription factor bi...
Gene manipulation, molecular cloning, genetic engineering Applications of DNA cloning Sequencing, hence to derive protein sequence; genomics Isolation and analysis of gene promoter etc Investigation of protein/enzyme/RNA function in various forms. Identification mutations, genetic diseases Biotechnology: ...
Numerous SDM techniques have been described over the last two decades, many of which involve more than one PCR step, the use of additional enzymes or further complex manipulations [23, 24]. We applied a SDM method based on in vivo homologous recombination using a single PCR step, which is ...
Further expression analysis is required to confirm these results, but finding similarly high EST counts between similar cell types supports the benefit of continuing these comparative approaches in more depth using RNA sequencing techniques. Stage-specific unigenes Figure 3 presented the overlap between ...
Gene expression data for Archaeplastida are accumulating exponentially, with more than 300 000 RNA-sequencing (RNA-seq) experiments available for hundreds of species. The gene expression data stem from thousands of experiments that capture gene expression in various organs, tissues, cell types, (a...
The discovery process often involves screening extracts or purified compounds from these bacteria against panels of bacteria and fungi, including drug-resistant strains. However, advanced techniques like genome sequencing have recently demonstrated that the chemical diversity captured by the traditional culture...