Gene sequencing technology and its applications in cancer precision medicine 基因测序肿瘤精准医学在临床肿瘤学中,基因突变检测在精准医学中起着关键作用.随着基因测序技术的进步,测序的成本和时间减少,其产量增加.基因测序技术可用于检测和分析癌症相关基因和潜在的分子机制.这些数据将帮助临床医生更好地为癌症患者制定...
Gene sequencing technology and its applications in cancer precision medicine作者:Mao-Hui Feng Wei Xie Xiao-Kang Wang 基本信息来源于合作网站,原文需代理用户跳转至来源网站获取 基因测序 肿瘤 精准医学 摘要: 在临床肿瘤学中,基因突变检测在精准医学中起着关键作用。随着基因测序技术的进步,测序的成本和时间减少...
fragments that form an “immune memory”. When bacteria are re-infected with the same phage, the CRISPR-Cas system destroys exogenous genes and enables the bacteria to acquire resistance to this phage. In 2012, Jinek et al. found that a single-guide RNA in the CRISPR-Cas system was able ...
the gRNAs are extracted from the cells, amplified via PCR, and their target genes are determined using next-generation sequencing.BOn the other hand, CRISPR mutagenesis screening begins with a gRNA library designed to induce in-frame mutations in the target protein coding sequence. After transducing...
Objective] To identifiy the etiologic agent from a paragonimiasis patient using molecular techniques. [Methosd] The complete nuclear ribosomal DNA second internal transcribed spacer (ITS2) gene sequence of eggs in sputum from a paragonimiasis patient was obtained by directly auto-sequencing its PCR pr...
Combining machine learning and single-cell sequencing to identify key immune genes in sepsis Hao Wang Linghan Len Yingchun Hu ResearchOpen Access10 Jan 2025Scientific Reports Volume: 15, P: 1557 The therapeutic potential of circular RNAs Circular RNAs have gained attention for their stability, immuno...
at the level of transcription, under specific circumstances or in a specific cell to give a global picture of cellular function. Techniques to measure this include DNA microarrays which measure the relative activity of previously identified target genes, or sequencing technologies that allow profiling ...
Traditionally, quantitative PCR has been used to measure the co-immunoprecipitation of specific target sequences with a transcription factor of interest. However, when combined with high-throughput sequencing techniques (ChIP-Seq) it is now possible to obtain an unbiased view of transcription factor bi...
The relationship between chromatin organization and gene regulation remains unclear. While disruption of chromatin domains and domain boundaries can lead to misexpression of developmental genes, acute depletion of regulators of genome organization has a
Gene expression is controlled by many simultaneous interactions, frequently measured collectively in biology and medicine by high-throughput technologies. It is a highly challenging task to infer from these data the generating effects and cooperating genes. Here, we present an unsupervised hypothesis-gener...