Gene sequencingCancerPrecision medicine在临床肿瘤学中,基因突变检测在精准医学中起着关键作用.随着基因测序技术的进步,测序的成本和时间减少,其产量增加.基因测序技术可用于检测和分析癌症相关基因和潜在的分子机制. 这些数据将帮助临床医生更好地为癌症患者制定合理的治疗方案,最终改善他们的预后.本综述将提供基因测序...
Gene sequencing technology and its applications in cancer precision medicine作者:Mao-Hui Feng Wei Xie Xiao-Kang Wang 基本信息来源于合作网站,原文需代理用户跳转至来源网站获取 基因测序 肿瘤 精准医学 摘要: 在临床肿瘤学中,基因突变检测在精准医学中起着关键作用。随着基因测序技术的进步,测序的成本和时间减少...
fragments that form an “immune memory”. When bacteria are re-infected with the same phage, the CRISPR-Cas system destroys exogenous genes and enables the bacteria to acquire resistance to this phage. In 2012, Jinek et al. found that a single-guide RNA in the CRISPR-Cas system was able ...
at the level of transcription, under specific circumstances or in a specific cell to give a global picture of cellular function. Techniques to measure this include DNA microarrays which measure the relative activity of previously identified target genes, or sequencing technologies that allow profiling ...
both with and without the drug. Activity-based cell sorting is used to enrich cells carrying mutations that make the drug ineffective, thereby identifying drug-resistant cells. Finally, the enriched cells are genotyped using deep sequencing to analyze structural changes and detect any escape mutants....
Based on such techniques, The Cancer Genome Atlas (TCGA) program has publicly shared a series of whole-genome sequencing data at different levels in 33 types of cancers [5]. To the best of our knowledge, HCC is a disease with a multifactorial aetiology, and hepatocarcinogenesis is a ...
1.3.1 Genes as Medicine Of the more than 1800 genetic disorders described in humans, only a small fraction can be treated and even fewer cured. At this time, we are seeing possible cures using gene therapy for sickle cell disease, thalassemia, and ADA deficiency. In a field with so many...
Gene expression data, such as RNA-sequencing or microarrays, usually suffer from the dimensionality problem: too many gene features and relative few samples. It is usually impractical to go through all of the features during the gene expression analysis. As a result, feature selection tends to ...
in particular for β-thalassemia. Recent advances in genome sequencing methods, improvements in gene delivery systems, understanding the molecular mechanism involved in regulation of theHBBlocus, and the progresses in gene-editing technology made a basis for new achievements in the treatment of hemoglobi...
In this study, we used the Bidirectional Elimination procedure, which is a combination of the forward and backward selection techniques. 2.6. Unsupervised Learning: Clustering Analysis Following the feature selection procedure, we studied the dataset homogeneity through an unsupervised learning method. ...