An isolated anti B type Clostridium botulinum neurotoxin monoclonal antibody having a neutralizing activity against B type botulinum neurotoxin including human monoclonal antibody and its antigen binding fragment are provided. In addition to hybridoma producing antibodies or fragments thereof, methods for ...
Broadly neutralizing antibodies (bNAbs) have been isolated from selected HIV-1-infected individuals and shown to bind to conserved sites on the envelope glycoprotein (Env). However, circulating plasma virus in these donors is usually resistant to autologous isolated bNAbs, indicating that during chronic...
MNT, as the reference method, employed living clade S and Delta viruses to measure neutralizing (NT) antibodies, while sVNT employed wild type strain and Delta receptor-binding domains (RBD) as the test antigens to measure sVNT antibodies. CMIA and ECLIA employed only one ...
Neutralizing human monoclonal antibodies binding multiple serotypes of botulinum neurotoxin.botulinumneurotoxinbotulismmolecularevolutionsingle-chainFvyeastdisplayBotulism, a disease of humans characterized by prolonged paralysis, is caused by botulinum neurotoxins (BoNTs), the most poisonous substances known. ...
Quantification of IgG antibodies that bind to rabies virus will not precisely demonstrate the level of protective virus neutralizing antibodies present in the sera and therefore the ELISA method is not appropriate for measuring the amount of RVNA present. Additionally, reporting of ELISA results in IU...
7, 8 Neutralizing antibodies can therefore play an important role in controlling human infection. The arenavirus surface glycoprotein complex (GPC) is the target of neutralizing antibodies. GPC comprises three functional subunits: the stable signal peptide (SSP), GP1, and GP2.9 GP1 binds cellular ...
have caused several outbreaks over the past two decades. The antigenic evolution of influenza viruses poses a significant challenge for the development of highly effective and long-lasting vaccines or antiviral drugs. Broadly neutralizing antibodies that target conserved epitopes can serve as prophylactic ...
To elucidate the efficient recognition sites by anti-bFGF neutralizing antibodies, we generated two anti-bFGF neutralizing monoclonal IgG1 antibodies (mAbs), 2G11 and 1E6, recognizing different sites, and estimated as binding to the heparin-binding and the receptor-binding regions of bFGF, ...
Here, we developed a panel of neutralizing anti-SARS-CoV-2 monoclonal antibodies (mAbs) that bound the receptor binding domain of the spike protein at distinct epitopes and blocked virus attachment to its host receptor, human angiotensin converting enzyme-2 (hACE2). Although several potently ...
All neutralizing monoclonal antibodies to VP5 * tested (YO-2C2, 2G4, 1A10) and two to VP7 (RV-3:2, RV-4:2) inhibited rotavirus binding to α2β1. Rotavirus binding to α4β1 was reduced by 2G4 and neutralizing antibody F45:2, directed to VP7. However, a neutralizing antibody ...