BHK-21IRF-1proliferation controlgrowth regulationexpressionsWe have established proliferation controled BHK cell lines employing IRF-1 (Interferon Regulatory Factor 1), a transcriptional activator that inhibits cell growth. The activity of IRF-1 has been made hormone dependent by fusing IRF-1 to the ...
Biochemical Analysis of Rabies Virus Proteins in Three Persistently Infected BHK-21 Cell Lines (1984) 上传人:成都理工·上传时间:2016-01-17
Fig.1 The comparison of culture morphology of three cell lines 2.2 批式培养过程中细胞生长曲线比较 以同一接种密度进行细胞培养,细胞生长曲线 (图2 )。 3种细胞的生长趋势基本相同。在0h～ 24h ,细胞处于潜伏期,细胞数目稍有增加;在培养 至24h～48h为指数生长期,细胞生长代谢旺盛,细 ...
細胞目錄-Cell Lines 細胞 遺傳變異細胞係和正常組織來源細胞... 美國Cell Marque 標準化的利器:電子式 IHC 蠟... Cell Marque IVD組... Manual Staining ... Hamilton Hamilton標準溶液 Hamilton移液器 Hamilton色譜柱 Hamilton稀釋儀 Hamilton進樣針/注射器... 菌株 菌種庫 微生物菌種 CMCC菌株 微...
The most frequently used mammalian host cell lines (BHK-21, CHO) for the production of recombinant therapeutic glycoproteins are incapable of producing α2,6-sialylated or sialyl Lewis X containing Oligosaccharide structures. Therefore, we have constructed novel stable BHK-21 cell lines by cotransfect...
The present study was undertaken to isolate and characterize Newcastle disease virus (NDV) from the outbreak of layer farms for the development of BHK-21 cell adapted inactivated vaccine. A total of 6 dead birds were brought to the laboratory from the outbreak area from which 18 samples (trache...
To investigate this further, we used two cell lines that have temperature-sensitive defects in genes essential for ubiquitin-dependent proteolysis: tsBN75 and ts41. tsBN75 is derived from cell line BHK and has a temperature-sensitive mutation in the ubiquitin-activating enzyme E1 (50). Cdt1 wa...
BHK 21 and VERO cell lines were used to compare the rLCMV ΔZ to the wild-type (WT) rLCMV in term of virus replication (at a multiplicity of infection [MOI] of 0.01). In term of virus propagation, results obtained with the rLCMV ΔZ and the WT virus were very similar using BHK-...
Cytotoxicity assay The sensitivity of cell lines to policresulen was investigated using an MTT assay. Briefly, BHK-21 cells were seeded into 96-well plates containing 100 μL of culture medium at a den- sity of 1×105 cells per well and were incubated overnight at 37 ˚C. Different ...
Safety Evaluation of Arsenicum album 6C in Cell Lines and BALB/c Mice Through Toxicity, Proliferation and Apoptosis Studies Wound healing and MTT assay showed that Arsenicum album 6C did not interfere with the normal rate of proliferation of BHK-21 cells. Moreover, DAPI ... R Anirban,S Avip...