Cell DivisionBHK 21/C 13 cells have been treated in maintenance medium with bullock thymus histones. This has produced a line of cells, which we have designated BHK 21/C 13/HT, capable of continuous sub-culture, and which shows virtually all the in vitro characteristics associated with ...
浓度,在同等条件下比较了3种不同来源的BHK-21悬浮细胞培养特性。结果显示,这3种细胞在形态上无 明显的差异,在显微镜下不易区分;在培养过程中, BHK-21-L细胞株在最短时间内可达到最大培养密度,细 胞生长速度快,细胞活性较高, DNA合成旺盛; BHK-21C13-2P细胞株最大密度较低,在72h～96h被完全 ...
BHK cell lines are used for various purposes including virus detection and production of virus and recombinant proteins in many laboratories throughout the world1 They also are valuable tools in the biomaterials surface characteristics studies. However,
Cell line name BHK/T7-9Comments Characteristics: Expression of the T7 RNA polymerase gene is under control of the strong chicken beta-actin promoter (PubMed=14524622).Transfected with: UniProtKB; P00573; Bacteriophage T7 DNA-directed RNA polymerase.Transfected with: UniProtKB; P00557; E.coli ...
The first recombinant human coagulation factor VIII of human origin: human cell line and manufacturing characteristics. Eur J Haematol. 2012;89:165–76. Article CAS Google Scholar Peters RT, Low SC, Kamphaus GD, Dumont JA, Amari JV, Lu Q, et al. Prolonged activity of factor IX as a ...
Background and Objectives: Several studies have been carried out on the use of cell lines in researches, production and processing of drugs and biological products, and on the identification of toxicity and efficacy.The present study was conducted to determine the characteristics of BHK-21 cell ...
Cell lineChromosomeMDBKThe established cell lines, Madian Darby Bovine Kidney (MDBK) and Baby Hamster Kidney (BHK-21 ) were used to find out the transformation of the chromosome numbers using G- banding, if any, in these cell lines which could be related to the number of passages given to...
None of these properties exactly parallels the differences observed in growth characteristics of the isolated variant cells.doi:10.1016/0042-6822(75)90146-4A. MeagerR. NairnR.C. HughesElsevier Inc.VirologyMeager A, Nairn R, Hughes RC. Analysis of transformed cell variants of BHK21 C13 isolated ...
A similar shift from a productive to a largely abortive response to Ad 2 was seen in BHK21 cells simultaneously infected with Ad 12 and Ad2. This finding supports the view that the restriction of Ad2 replication in Ad 12-transformed cells, like the other characteristics mentioned, is ...
this cell line is then produced by developed rabies virus in quantities dovoluj\\u00edc\\u00edm production antirabick\\u00fdch vaccines.during the cultivation of the rabies virus does not change its basic characteristics from the point of view of safety and immunogenicity after administration of ...