The precursor of Escherichia coli RTEM beta-lactamase was purified to homogeneity on a milligram scale by a procedure independent of the binding properties of the protein and refolded to an active, reduced form. For comparing the folding kinetics, the wild-type enzyme was reduced and a mutant ...
CONJUGATES OF A CEPHALOSPORIN DERIVATIVE AND A FLUOROPHOR PRECURSOR AND RELATED COMPOUNDS AS SUBSTRATES FOR BETA-LACTAMASE GENE EXPRESSIONFluorescent substrates for beta-lactamases having the general formula shown above are provided. Z includes a fluorophore or chromophore and includes a group that may ...
Conjugates of a cephalosporin derivative and a fluorophor precursor and related compounds as substrates for beta-lactamase gene expressiondoi:WO2006085978 A2Fluorescent substrates for beta-lactamases having the general formula shown above are provided. Z includes a fluorophore or chromophore and includes a...