The precursor of Escherichia coli RTEM beta-lactamase was purified to homogeneity on a milligram scale by a procedure independent of the binding properties of the protein and refolded to an active, reduced form. For comparing the folding kinetics, the wild-type enzyme was reduced and a mutant ...
A less common plasmid-encoded class A β-lactamase is SHV-1. This enzyme shares about 60% identity with TEM-1 and has a similar substrate profile. It also has been a precursor ofESBLsand started to mutate. The pivotal mutation was an alteration at codon 238 converting glycine to serine....
1990. The Escherichia coli heat shock proteins GroEL and GroES modulate fold- ing of the -lactamase precursor. EMBO J. 9:2315-2319.Laminet, A., Ziegelho¡er, T., Georgopoulos, C. and Pluckthun, A. (1990) The Escherichia coli heat shock proteins GroEL and GroES modulate the ...
CONJUGATES OF A CEPHALOSPORIN DERIVATIVE AND A FLUOROPHOR PRECURSOR AND RELATED COMPOUNDS AS SUBSTRATES FOR BETA-LACTAMASE GENE EXPRESSIONFluorescent substrates for beta-lactamases having the general formula shown above are provided. Z includes a fluorophore or chromophore and includes a group that may ...
Conjugates of a cephalosporin derivative and a fluorophor precursor and related compounds as substrates for beta-lactamase gene expressiondoi:WO2006085978 A2Fluorescent substrates for beta-lactamases having the general formula shown above are provided. Z includes a fluorophore or chromophore and includes a...
(PBS); class A β-lactamase from Klebsiella pneumoniae was initially thought to be a “sulfhydryl variant” of the TEM enzyme (SHV); 3-nitrophenyl boronic acid (3-NPBA); tris[(1-benzyl-1H-1,2,3-triazole-4-yl)methyl]amine (TBTA); Verona integron-encoded metallo-β-lactamase (VIM); ...
A similar finding was reported in an experimental Enterobacter strain where a mutation in SHV-12 (Ambler class A) β-lactamase reduced the susceptibility to aztreonam-avibactam by significantly impairing avibactam inhibitory binding [38]. 3.4. Clinical Experience with Aztreonam-Avibactam Clinical ...