The precursor of Escherichia coli RTEM beta-lactamase was purified to homogeneity on a milligram scale by a procedure independent of the binding properties of the protein and refolded to an active, reduced form. For comparing the folding kinetics, the wild-type enzyme was reduced and a mutant ...
1985. Precursor of P-lactamase is enzymatically inactive: accumula- tion of the preprotein in Saccharomyces cerevisiae. J. Biol. Chem. 260:1508-1512.Roggenkamp R, Dargatz H, Hollenberg CP (1985) Precursor of fl-lactamase is enzymatically inactive: accumulation of the pre- protein in ...
A 1400-base DNA fragment, which contains the gene encoding the extracellular active-site serine -lactamase of Streptomyces albus G previously cloned into Streptomyces lividans [Dehottay et al. (1986) Gene 42 , 3136], was sequenced. The gene codes for a 314-amino-acid precursor, the N-...
In embodiments, the beta-lactam ring may be cleaved by a beta-lactamase enzyme effective to free a fluorophore. Methods of assaying beta-lactamase activity and monitoring expression in systems using beta-lactamase as a reporter gene are also disclosed....
In embodiments, the beta-lactam ring may be cleaved by a beta-lactamase enzyme effective to free a fluorophore. Methods of assaying beta-lactamase activity and monitoring expression in systems using beta-lactamase as a reporter gene are also disclosed.Jianghong Rao...