The precursor of Escherichia coli RTEM beta-lactamase was purified to homogeneity on a milligram scale by a procedure independent of the binding properties of the protein and refolded to an active, reduced form. For comparing the folding kinetics, the wild-type enzyme was reduced and a mutant ...
1985. Precursor of P-lactamase is enzymatically inactive: accumula- tion of the preprotein in Saccharomyces cerevisiae. J. Biol. Chem. 260:1508-1512.Roggenkamp R, Dargatz H, Hollenberg CP (1985) Precursor of fl-lactamase is enzymatically inactive: accumulation of the pre- protein in ...
CONJUGATES OF A CEPHALOSPORIN DERIVATIVE AND A FLUOROPHOR PRECURSOR AND RELATED COMPOUNDS AS SUBSTRATES FOR BETA-LACTAMASE GENE EXPRESSIONFluorescent substrates for beta-lactamases having the general formula shown above are provided. Z includes a fluorophore or chromophore and includes a group that may ...
In embodiments, the beta-lactam ring may be cleaved by a beta-lactamase enzyme effective to free a fluorophore. Methods of assaying beta-lactamase activity and monitoring expression in systems using beta-lactamase as a reporter gene are also disclosed.Jianghong Rao...