principle of the bicinchoninic acid (BCA) assay is similar to the Lowry procedure,1in that both rely on the formation of a Cu 2+-protein complex under alkaline conditions, followed by reduction of the Cu 2+to Cu 1+. The amount of reduction is proportional to the protein present. It has...
Proteindeterminationisoneofthemostcommon operationsperformedinbiochemicalresearch.The principleofthebicinchoninicacid(BCA)assayissimilar totheLowryprocedure, 1 inthatbothrelyonthe formationofaCu 2+ -proteincomplexunderalkaline conditions,followedbyreductionoftheCu 2+ toCu 1+ . Theamountofreductionisproportional...