蛋白质在碱性条件下将二价铜离子还原为一价铜离子,一价铜离子与BCA 分子形成紫色络合物。此络合物可用540-590 nm波长检测,反应物的颜色和蛋白浓度在一定范围内具有线性关系。 产品名称 英文名称 货号 BCA 蛋白浓度测定试剂盒 BCA Protein Concentration Assay Kit ...
[translate] aProtein concentration was determined by the BCA protein assay reagent kit (Pierce), and bovine serum albumin was used as a standard. 正在翻译,请等待...[translate]
BCA Protein Assay Kit is based on one of the most popular protein concentration detection methods: BCA (bicinchoninic acid) method. The principle is the peptide bond of protein reacts with Cu2 + to form a complex, and Cu2 + is reduced to Cu + in alkaline environment. Then the BCA reage...
蛋白质浓度的测定是常见的生物实验之一。本文介绍的是使用BCA法(二辛可酸法或二喹啉甲酸法)进行蛋白质浓度的测定。 一、实验原理 BCA是一种稳定的碱性水溶性复合物。在碱性条件下,蛋白质可以将BCA试剂中的二价铜离子Cu2+还原成一价铜离子Cu+,接着还原出来的Cu+与BCA试剂螯合形成紫蓝色络合物,该络合物在562nm处...
BCA assayCompat-Able Protein AssaygelatinNHS interferenceConducting the bicinchoninic acid (BCA) assay directly after a coupling reaction using (1-ethyl-3-(3-dimethylaminopropyl) carbodiimide) (EDC) and N-hydroxysuccinimide (NHS) chemistry produces significant errors. Here we present a correction for ...
产品名称:Pierce™ BCA Protein Assay Kit 描述: The Thermo Scientific Pierce BCA Protein Assay Kit is a two-component, high-precision, detergent-compatible assay reagent set to measure (A562nm) total protein concentration compared to a protein standard. ...
23227PierceBCAProteinAssayKit,sufficientreagentsfr250test-tuber2500micrplateassays KitContents: BCAReagentA,1000m(inPrductN.23225)r500m(inPrductN.23227),cntaining sdiumcarbnate,sdiumbicarbnate,bicinchninicacidandsdiumtartratein0.1Msdium hydrxide ...
BCA protein concentration determination kit (with accessories: BSA standard 1ML×20)|BCA法蛋白浓度测定试剂盒(带附件:BSA标准品 1ML×20支) 基本信息 更多信息 中文名称: BCA法蛋白浓度测定试剂盒(带附件:BSA标准品 1ML×20支) 中文同义词: BCA法蛋白浓度测定试剂盒(带附件:BSA标准品 1ML×20支) 英文...
中文名称: BCA蛋白浓度测定试剂盒(500T) 中文同义词: BCA蛋白浓度测定试剂盒(500T) 英文名称: BCA Protein Concentration Kit (500T) 英文同义词: CAS号: 分子式: 分子量: 0 EINECS号: Mol文件: Mol File BCA蛋白浓度测定试剂盒(500T) 信息错误报告 提交 按...
The invention discloses an enhanced type protein concentration assay kit by use of BCA (bicinchonininc acid). A kit body is made of a rigid plastic material, a cover is connected on the kit body, a reagent gasket made of a rigid plastic material is arranged in the kit body, a first ...