The bicinchoninic acid (BCA) assay for protein quantitation. Methods Mol. Biol.Walker JM. The bicinchoninic acid (BCA) assay for protein quantitation. The Protein Protocols Handbook: Springer; 2009. p. 11-15.Walker JM. The bicinchoninic acid (BCA) assay for protein quantitation. Methods Mol ...
absorb-ance maximum at 562 nm. Since the production of Cu+ in this assay is a function of protein concentration and incubation time, the protein content of unknown samples may be determined spectrophotometrically by comparison with known protein standards. A further advantage of the BCA assay is...
BCA 测蛋白浓度的原理 BCA 全称是Bicinchoninic Acid Assay, 也叫Smith Assay,因为这个方法是Paul K. Smith 发明的……这个方法通过展示不同浓度样品的颜色变化来判断蛋白的浓度。 图片源自网络 BCA分析主要依靠两个反应。首先,蛋白质中的肽键可将硫酸铜中的Cu2+还原成Cu +(反应依赖于温度),其中减少的Cu 2+的量...
Since the production of Cu+ in this assay is a function of protein concentration and incubation time, the protein content of unknown samples may be determined spectrophotometrically by comparison with known protein standards. A further advantage of the BCA assay is that it is generally more ...
Since the production of Cu(I) in this assay is a function of protein concentration and incubation time, the protein content of unknown samples can be determined spectrophotometrically by comparison with known protein standards. A further...
BackgroundAbbkine Protein Quantification Kit (BCA Assay) is improved on the basis of BCA method, one of the commonly used protein concentration detection methods in the world. The principle of this method is that the protein reduces copper ions (Cu2+) to cuprous ions (Cu+ ) in alkaline cond...
The Pierce BCA Protein Assay Kit is a high-precision, detergent-compatible protein assay for determination of protein concentration. Pierce BCA reagents provide accurate determination of protein concentration with most sample types encountered in protein research.Have...
蛋白质在碱性条件下将二价铜离子还原为一价铜离子,一价铜离子与BCA 分子形成紫色络合物。此络合物可用540-590 nm波长检测,反应物的颜色和蛋白浓度在一定范围内具有线性关系。 产品名称 英文名称 货号 BCA 蛋白浓度测定试剂盒 BCA Protein Concentration Assay Kit ...
(BCA)proteinassayreagent.Inonesense,itisamodificationoftheLowryproteinassayreagent.ThemechanismofcolorformationwithproteinfortheBCAproteinassayreagentissimilartothatoftheLowryreagent,butthereareseveralsignificantdifferences.TheBCAproteinassayreagentcombinesthereductionofCu 2+toCu+byproteininanalkalinemedium(i.e.,...
Since the production of Cu+ in this assay is a function of protein concentration and incubation time, the protein content of unknown samples may be determined spectrophotometrically by comparison with known protein standards. A further advantage of the BCA assay is that it is generally more ...