Walker JM: The bicinchoninic acid (BCA) assay for protein quantitation. Methods Mol Biol 32: 5‑8, 1994.Walker, J. M. 1996. The bicinchoninic acid (BCA) assay for protein quantitation. In The Protein Protocols Handbook, 11-14. Totowa, N.J.: Humana Press....
The Bicinchoninic Acid (BCA) Assay for Protein Quantitation John M. Walker 1. Introduction The bicinchoninic acid (BCA) assay, first described by Smith et al. (1) is similar to the Lowry assay, since it also depends on the conversion of Cu2+ to Cu+ under alkaline conditions (see Chapter...
for 0.5-30 μg/ml protein Synonym(s): BCA Assay, Protein determination by BCA, bicinchoninic acid reagent NACRES: NA.84Recommended Products Slide 1 of 6 328693 ALNOCHROMIX™ View Price and Availability Sigma-Aldrich ET0100 E-Toxate™ Kit View Price and Availability Supelco MSCAL3 Proteo...
Fig. Abbkine Protein Quantification Kit (BCA Assay) standard curve. Tips for Abbkine antibodies 1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range...
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与 Bradford法或 Pierce BCA Protein Assay 试剂盒相比,免稀释Rapid Gold BCA 蛋白定量试剂盒无需稀释样品和标准品,从而可将实验时间减少高达 80%。 此外,它只需要 5 分钟的室温孵育。 经典BCA试剂盒也有内含免稀释 BSA 蛋白标准品的版本—— Pierce 免稀释BCA Protein Assay 试剂盒。 图3. 与其他蛋白质...
Copper-based total protein quantification The BCA Protein Assay is a popular method for colorimetric detection and quantitation of total protein. Pierce BCA Protein Assays have a unique advantage over the Coomassie dye–based assays (Bradford)–they are compa...
BCA Protein Assay Kit 是目前世界上最常用的两种蛋白浓度检测方法之一。BCA 蛋白质定量包括两步反应:首先,二价铜离子 (Cu2+) 在碱性条件下被蛋白质的肽键还原成一价铜离子 (Cu+);其次,两个分子的 BCA 络合一个一价铜离子 (Cu+),形成一种在 562 nm 处有强吸收值的紫色复合物,而复合物的吸收值与蛋白的...
The QuantiPro™ BCA Assay Kit is used to determine protein concentrations in the range of 0.5-30 μg/mL.
英文名BCA Protein Assay Kit 相关类别蛋白定量储存按标签提示分开储存 编 号包装库存目录价(￥)您的价格(￥)数量 C503021-0500500 ASSAYS现货520520 C503021-0501500 ASSAYS+5 MICRO现货612612 产品描述 概述 BCA法是理想的蛋白质定量方法,在碱性环境下蛋白质分子中肽键结构与Cu2+络合并将Cu2+还原成Cu1+。BCA特...