fluorescent proteins can be observed as a signal using an analyzer that is also parallel to the excitation light polarization vector (green wave). The resulting anisotropy, which is an indicator of the degree of orientation, can be determined by measuring and comparing the emission intensity ...
Visible fluorescent proteins (VFPs) have been widely used as a FRET pair in addition to organic dyes. Light microscopy techniques including wide-field, confocal and multiphoton microscopy systems provide spatial information of the interacting proteins with nanometer resolution. For better interpretation ...
Visible fluorescent proteins (VFPs) have been widely used as a FRET pair in addition to organic dyes. Light microscopy techniques including wide-field, confocal and multiphoton microscopy systems provide spatial information of the interacting proteins with nanometer resolution. For better interpretation ...
stand and/or stretch, and allow the eyes to focus at a distance. Objects that must be accessed frequently should be kept close enough to avoid having to stretch and strain, usually within a distance of 9-19 inches. Less frequently utilized objects can be kept at a distance of 9-25 ...
Keller: Basics of a Light Microscopy Imaging System and Its Application in Biology (Oxford University Press, New York, 2001) Methods in Cellular Imaging.L. Davidson and R. Keller: Basics of a Light Microscopy Imaging System and Its Application in Biology (Oxford University Press, New York, ...
Figure 3. An image of the same field of BPAE cells captured using brightfield (left) and fluorescence (right) microscopy. Fluorescent labeling of the nucleus (yellow) and actin (red) makes it possible to see much more detail...
Fluorescent Specimens-Cells in tissue culture, fluorochrome-stained sections, smears and spreads Fluorescence Illumination Birefringent Specimens-Mineral thin sections, liquid crystals, melted and re-crystallized chemicals, hairs & fibers, bones & feathers ...
Fujiwara K, Pollard TD (1976) Fluorescent antibody localization of myosin in the cytoplasm, cleavage furrow, and mitotic spindle of human cells. J Cell Biol 71:848–875 PubMedCASGoogle Scholar Shewan AM, Maddugoda M, Kraemer A, Stehbens SJ, Verma S, Kovacs EM, Yap AS (2005) Myosin 2...
methodsto fix this problem. Whereasphase contrast(PH) anddifferential interference contrast(DIC) manipulate the light passing through the specimen to add contrast, you can also stain it withfluorescent dyes(How to Prepare your Specimen for Immunofluorescence Microscopy) respectively usefluore...
For most nucleic acid stains the fluorescent signal is minimal before binding to nucleic acids, and there is a significant increase in fluorescence intensity after the dye has bound the nucleic acid. Find general instructions for labeling the nuclei ...