Introduction to Optics and Photophysics (Rainer Heintzmann) Principles of Light Microscopy (Ulrich Kubitscheck) Fluorescence Microscopy (Jurek W. Dobrucki) Fluorescence Labeling (G. Ulrich Nienhaus, Karin Nienhaus) Confocal Microscopy (Nikolaus Naredi–Rainer, Jens Prescher, Achim Hartschuh, Don...
CW solid state lasers for fluorescence microscopy: 405 nm - 660 nm, up to 250 mW. Compact individual lasers or multi-line lasers by Cobolt using HTCure.
through stimulated emission. STED microscopy provides a smaller probing volume compared to confocal microscopy, because only the fluorophores near the center of the doughnut beam (below theI0intensity threshold for stimulated emission, dashed line) are allowed to remain in the excited state and fluores...
The combination of confocal laser-scanning microscopy (CLSM) and fluorescence fluctuation spectroscopy (FFS) is a powerful tool in studying fast, sub-resolution biomolecular processes in living cells. A detector array can further enhance CLSM-based FFS techniques, as it allows the simultaneous acquisiti...
Confocal microscopy Confocal fluorescence microscopy was performed on a Leica SP8 FALCON microscope (Leica Microsystems) equipped with a Leica TCS SP8 X scanhead; a SuperK white light laser, Leica HyD SMD detectors, a HC PL APO CS2 ×20/0.75 dry objective, a HC PL APO CS2 ×40/1.10 water...
The two main fluorescence microscopy configurations that are used nowadays are wide‐field fluorescence microscopy (WFM) and confocal laser scanning microscopy (CLSM). In WFM the sample under study is evenly illuminated with the collimated, infinity‐focused light beam. Unlike WFM, in CLSM the ...
Lightpath aberrations are those that actually occur within the microscope system you are using to image your sample. There are 3 main causes of aberrations that different types of objectives can correct.https://www.microscopyu.com/microscopy-basics/introduction-to-microscope-objectives1. Chromatic aber...
reduction of QA, Chl fluorescence,F, rises from the O level (the minimal level) to the P level (the peak); yet, the relationship betweenFand [QA−] is not linear, due to the presence of other quenchers and modifiers. Several alternative theories have been proposed, which give different...
Microscopy 3.3 Confocal Optical Microscopy 4 Applications 4.1 Protein Folding 4.1.1 The Unfolded State 4.1.2 Single Molecule Folding/Unfolding Transitions 4.2 Enzymes 4.2.1 Static and Dynamic Heterogeneity 4.2.2 Conformational Changes and Catalytic Activity 5 Conclusions Keywords: fluorescence light; ...
Sensitized acceptor emission FRET microscopy. Cells were investi- gated on a Zeiss confocal LSM 510 META microscope using a 40Â NA 1.2 C-Apochromat objective. Sensitized acceptor emission FRET measurements and analysis were performed according to the ...