How do you increase your resolution? Add in fluorophores to stain structures in your sample and filters to illuminate it, focus the light emitted by the sample, add a sensitive detector, and voila! Now you can do fluorescence ...
IF preparations can be analyzed by various microscopy techniques (e.g. CLSM, Epifluorescence,TIRF,GSDIM), depending on the application or the researcher’s interest. Meanwhile, IF has become indispensable for a large number of research groups which have at least access to a simple fluoresc...
How to optimize TIRF scans.(Microscopy)(Total Internal Ieflectance Fluorescence)(Brief Article)Kramer, Matthias
a7. If antibody responses are robust, evaluate ADCC, CDC, internalization (fluorescence microscopy) and degradation of CEA (Western blot).. 7. 如果抗体反应是健壮的,评估ADCC、CDC、内在化 (CEA) 西部污点的荧光显微法和 (退化)。[translate]
In this edition of HowStuffWorks, we will enter the tiny world of light microscopes and examine the various technologies that let them expose what is otherwise undetectable to the human eye. Contents The Basics Image Quality Types of Microscopy Fluorescence Microscopy The Parts of a Light ...
Single-molecule microscopy has evolved into the ultimate-sensitivity toolkit to study systems from small molecules to living cells, with the prospect of revolutionizing the modern biosciences. Here we survey the current state of the art in single-molecule tools including fluorescence spectroscopy, tethere...
to capture several fluorescence imaging channels simultaneously for their exact spatiotemporal correlation. Mica enables users to acquire the signal of up to four fluorophores at a time, either in confocal or widefield mode. With it, cellular components can be imaged at the very same point in...
In traditionalfluorescence microscopy, one photon of light of a specific wavelength excites a fluorophore molecule in the sample, leading to fluorescence emission. Two photon microscopy utilises two photons of half the energy (and thus twice the wavelength), that interact with the fluorophore within ...
Alternatively, fluorescence polarisation can be used to determine RdRP activity by its complementation of a template RNA strand. If fluorescently labelled single-strand RNA is not elongated, it shows a low polarization value. The RNA molecule increases its size, if the complementary strand is synthesi...
N.B.:Leica Microsystems does not make any statement as to the suitability of sodium hypochlorite and other chlorine releasing compounds used in connection with Leica microscopes, as their use is outside of the manufacturer´s recommendations ...