The effect of the expression level of B7-H3 and B7-H1 proteins in the tumors of patients with prostate cancer had not yet been investigated. They have now shown that B7-H3 predicts prognosis following surgery for prostate cancer.doi:10.1038/ncpuro0923None...
BJU Int 100: 392–395 B7-H3 expression level predicts prostate cancer progression following surgery Roth et al. previously reported that aberrant expression of the T-cell inhibitory ligand B7-H1 in the tumors of patients with renal cell carci- noma predicted aggressive disease and shorter survival...
Protein-level expression profile of B7-H3 indicates that posttranscriptional and posttranslational regulations are essential for its expression, and the effect of modulating B7-H3 expression on BC is progressively being investigated. Through interacting with the 3'-untranslated region of B7-H3, miR-29...
In the present study, we explored the expression level of B7-H3 and ICAM-1 in different types of thyroid carcinoma. In the groups of the TCGA cohort, both B7-H3 and ICAM-1 mRNA were highly expressed in thyroid carcinoma. Furthermore, the patients with Stage2, 61-80y, Follicular thyroid...
C Flow cytometry analysis was performed to determine B7-H3 expression in U87-MG, TJ905, and HTB15 cells. The protein expression of B7-H3 was 92.6%, 91.8%, and 93.2% in U-87MG, TJ905, and HTB15, respectively. D Vγ9Vδ2 T or Car-B7H3-γδT cells were incubated with U-87MG-...
Notes: (A) B7-H3 mRNA expression was measured by RT-PCR. Lane 1: Cells were treated with siRNA of control. Lane 2: Cells were treated with siRNA of B7-H3 for 48 hours. Lane 3: Marker DL2000. (B) Expression level of B7-H3 mRNA was measured by means of real-time PCR 48 hours ...
ORR was reported in 35% out of the 40 response-evaluable patients, regardless of baseline B7-H3 expression level, with a disease control rate of 85.0%. In the subset of 11 evaluable SCLC patients, the ORR was observed in 63.6% of cases, all occurring at the first disease assessment with...
Figure 2. B7-H3 Expression Level in Primary GBM Cells and GBM Cell Lines (A and C) Immunofluorescence staining indicated the expression of B7-H3 (red) on cell surface in GBM primary cells (A) and glioma cell lines (C). (B and D) Consistent with the immunofluorescence result, the flow...
The expression level of the housekeeping gene β2-microglobulin was measured as an internal reference with a standard curve to determine the integrity of template RNA for all specimens. The ratio of the mRNA level of each gene was calculated as follows: (absolute copy number of each gene)/(...
We have previously reported overexpression of the immunoregulatory protein B7-H3 in colorectal cancer and that nuclear expression predicted poor outcome in colon cancer patients. The present study was performed to examine the prognostic role of B7-H3 in an independent colorectal cancer cohort. Methods ...