The aim of this work is to explore the anti-melanoma potential of maltol through regulating immune checkpoints. Methods: The immune checkpoint PD-L1 was analyzed using qPCR, immunoblots, and immunofluorescence. Melanoma sensitivity towards T cells was investigated via cytoto...
【Key words】PDL1；Stable expression；B16F10cell lines 细胞程序性死亡配体1（Programmed cell death ligand1，PD－L1）属于B7超家族成员，主要表达在B 细胞、巨噬细胞等抗原提呈细胞表面［1］。PD－L1的受体为PD－1，其胞质区含有免疫受体酪氨酸抑制基序，为抑制性的受体。静止的T细胞不表达PD－1受体，T细胞...
益生菌对基于PD-1免疫疗法治疗小鼠黑色素瘤的影响及作用机制研究 热度: 南京中医药大学 硕士学位论文 盐酸川芎嗪影响B16F10黑色素瘤转移的机制研究 姓名:*** 申请学位级别:硕士 专业:药理学 指导教师:** 20080501 中文摘要 盐酸川芎嗪影响B16F10黑色素瘤转移 的...
They also reported blocking of HMGB1 improved efficacy of anti-PD-1 therapy. Induction of Treg by HMGB1 is also reported in type 1 diabetes [21] and psoriasis vulgaris, an autoimmune inflammatory skin disease [22]. Induction of MDSCs and regulatory B cells by HMGB1 have been reported [23,...
Seven tumor-bearing mice from each group were monitored for the anti-tumor effects of treatment with different formulations. The administration of liposomal V1 and V3 peptides considerably slowed the growth of the tumor and in...
SK-Mel-28 cells pre-treated with jara, and subsequently injected in mice selected for anti-inflammatory response significantly reduced the number of lung metastases [21]. In the present study, the decreased tumor volume, and the increased number of treated cells in G0/G1 and arrest in G2/M...
Taken together, these results suggest that ZER and ZO extract can be used as active ingredients in skin-whitening cosmetics because of their anti-melanogenic effect. Keywords: zerumbone; Zingiber officinale roscoe; melanogenesis; MITF; ERK1/2 1. Introduction Melanogenesis, the production of melanin...
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A specific ERK inhibitor PD98059 significantly blocks OPA-inhibited melanin synthesis and tyrosinase activity, whereas a p38MAP and JNK inhibitor had no effect. These findings provide evidence demonstrating that the anti-melanogenic effect of OPA is mediated through the activation of ERK signal pathway...
distribution into FACS tubes, followed by incubation with either anti-mouse CD68-PE/CD86-APC or CD68-PE/CD163-Brilliant Violet 421 antibodies. Based on gated CD68-positive cells, the M1 and M2 phenotype of macrophages was determined with a BD LSR II flow cytometer using the Diva 6.1.1. ...