of the b-Globin Gene LocusChakalova, LyubomiraCarter, DavidDebrand, EmmanuelGoyenechea, BeatrizHorton, AliceMiles, JoanneOsborne, Cameron
The distribution of a nucleotide polymorphism in intron 2 of the β-globin gene (IVS-2 nt 666 C > T was examined in populations in southern Germany and Cameroon. The allelic frequencies were 0.86 for T and 0.14 for C in southern Germany and 0.87 for T and 0.13 for C in Cameroon, resp...
该研究通过基因编辑技术编辑β-地中海贫血患者的造血干细胞中的BCL11A增强子位点,并进行自体造血干细胞移植,编辑后的造血干细胞可以分化为含有高水平HbF的红细胞,有望彻底根治由β-globin珠蛋白突变引发的系列遗传疾病。 目前邦耀生物团队正...
pSPORT1[NheI/glGFP/bglobin3'-UTR/Ins2] catalog : 74101 more info or order: Addgene product webpage citations: 1 Reference Wang C, Szaro B. A method for using direct injection of plasmid DNA to study cis-regulatory element activity in F0 Xenopus embryos and tadpoles. Dev Biol. 2015;39...
Keywords Multiplex genotyping Æ Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry Æ b-globin gene Æ Minisequencing Æ b-thalassemia Introduction b-thalassemia, a common hereditary disease found throughout the world, is caused by the mutation of a single gene (...
也可以在末端平滑化和磷酸化后克隆到平滑末端载体。 ■ PCR反应性能 1. 以λ DNA为模板,可以很好地扩增20 kb的DNA片段。 2. 以人基因组DNA为模板,可很好地扩增17.5 kb (β-Globin gene) 的DNA片段。 维护中,请联系客服 维护中,请联系客服
The expression of the human β-like globin genes follows a well-orchestrated developmental pattern, undergoing two essential switches, the first one during the first weeks of gestation (ε to γ), and the second one during the perinatal period (γ to β). The γ- to β-globin gene switchin...
Whereas, the coregulation of a and b globin gene during RBC development is well established, there are multiple transcription factor binding motifs in the b-globin gene promoter that are absent from that of a-globin19. We have shown that the Kruppel-like transcription factor KLF4, which binds...
Gene transfer in humans using a conditionally replicating lentiviral vector. Proc Natl Acad Sci USA 2006; 103: 17372–17377. 16 Bank A, Dorazio R, Leboulch P. A phase I/II clinical trial of beta- globin gene therapy for beta-thalassemia. Ann NY Acad Sci 2005; 1054: 308–316. 17 ...
The expression vector of the invention also contains a polyadenylation site downstream from the structural gene of a non-A non-B hepatitis-specific antigen. Illustrative examples of the polyadenylation sites may include those derived from SV40 DNA, β-globin gene or methallothionein gene. A combine...