细胞自噬相关蛋白(autophagy-related gene- product,ATG)8-磷脂酰乙醇胺(phosphatidylethan olamine,PE)是迄今唯一定位于细胞自噬体膜上 的蛋白质,与细胞自噬体的形成密切相关,是检 测细胞自噬的良好标志物。 细胞自噬相关蛋白8及其连接系统与头颈部恶性肿瘤 [摘要] 细胞自噬相关蛋白(ATG)8-磷脂酰乙醇胺(PE)是...
One study of a family with autosomal dominant DMRV, identified the c.1165 + 1 G > A splice donor variant in the SQSTM1/p62 gene in the affected individuals but also in one non-family related patient [126]. Interestingly, DMRV patients also have SQSTM1/p62 inclusions [126]. ...
218, 219 Interestingly, FBX07, a protein part of the SKP1-CUL1-RBX1 ubiquitin ligase complex and encoded by the PD-related gene PARK15, was reported to act in a common pathway with PINK1 and Parkin.220 Recent evidence, however, points to a non-essential role of FBXO7 in this mitophagy...
On the basis of gene expression signatures based on autophagy-related genes (ARGs), several cancers such as colon cancer21, breast cancer22, ovarian cancer23, and non-small-cell lung cancer24have been associated with gene expression signatures. An ARG-based prognostic model for pancreatic cancer ...
As genes associated with the autophagy pathway genes regulating autophagy are poorly annotated in ontology databases we applied an in-house gene list (244 genes referred as ‘Autophagy pathway’) (Moussay et al, 2011), revealing strong modulation of the autophagy pathway upon both short- and ...
Background: Autophagy is a self-digesting process that can satisfy the metabolic needs of cells, and is closely related to development of cancer. However, the effect of autophagy-related genes (ARGs) on the prognosis of breast cancer remains unclear.<
Equally, the effects of other cell and tissues types and their related secretions on cartilage and bone cells cannot be recaptured within a unicellular system. Whilst a number of different animals have been used as models for bone research, mouse models remain the most extensively used due to ...
Either kit can be used to continuously measure the activity of caspase-3 and closely related proteases in cell extracts and purified enzyme preparations using a fluorescence microplate reader or fluorometer. AMC-based DEVD substrates, which yield blue fluorescen...
Autophagosome nucleation begins with the activation of several autophagy-related gene (ATG) proteins, with the help of VPS34 and BECLIN1. A double-membraned isolation membrane elongates and forms around the ubiquitinated substrate of interest. Adaptor proteins, like p62 (also known as sequestosome 1...
The initiation of autophagy is controlled by Unc-51-like kinase 1 (ULK1) complex, which is comprised of the serine/threonine-protein kinase ULK1, family kinase-interacting protein 200 (FIP200), autophagy-related gene 13 (Atg 13) [40]. Upon autophagy stimuli, such as amino acid starvation ...