42. We fed rapamycin at doses between 50 μM and 400 μM and measured cell size after 14 days (Fig.2c). Enterocyte size in untreated males was significantly smaller than in untreated females, as expected
Mosaic Dendra-tau fish were imaged before and after photoconversion and then daily, to assess clearance of the red (photoconverted) fluorescently tagged tau protein (Supplementary Fig. 6a,b). The autophagy-enhancing drugs rapamycin, clonidine and rilmenidine led to decreased abundance of red Dendra...
Drinking ACV can help you feel full longer and reduce your appetite. It helps to slow digestion when paired with starchy foods. This helps to stabilize your insulin levels and make you feel full while digesting. Try 1-2 tablespoons in water before meals or as a morning primer. You can als...
Five-day-old WT seedlings under the conditions as above-mentioned were treated with dark for 0, 6, 12, and 24 h before harvesting for RNA extraction. UBQ10 was used as an internal control. Dark-induced DIN6 expression was used as a positive control. The expression level at 0 h was ...
Disease development was monitored weekly by measuring the footpad swelling (a) and lesion size (b). The pictures in c show the lesion at week 5 post-infection. The results represent the mean ± SD of two independent experiments, with 6 mice per group. Mice were euthanized when the ...
Aging is an important pathogenic factor of age-related diseases and has brought huge health threat and economic burden to the society. Dendrobium nobile Lindl., a valuable herb in China, promotes longevity according to the record of ancient Chinese mater
Intracellular components to be degraded through activation of macroautophagy are first engulfed in double-membrane vesicles, named autophagosomes, before being fused with the lysosomal membrane and eventually cleared [34]. In humans, the microtubule-associated protein light chain 3 (LC3) is generated ...
The colonies were then counted and taken pictures. Wound healing Using a 200 µl pipette tip, a scratch was created in each well once the cells had reached approximately 90% confluency. The tip was maintained in alignment with the plate. Before being cultured in DMEM media without FBS, ...
Before intervention, a 2 mm thick coupler (Subijie, Chongqing, China) was applied between the bottom of the plate and the ultrasound probe. After exposed to LIPUS, chondrocytes were harvested immediately. In the meantime, the control group was put in the identical environment but except LIPUS ...
Membranes were washed 3 times 10 min with TBS after incubation with primary and secondary antibodies. Pictures were acquired using West Femto ECL (34096 Thermofischer), with PXI4 (Syngene), GeneTools (Syngene) or Odyssey Fc (LICOR). Quantifications were performed using ImageJ. For fluorescent...