Many aspects of these autophagy-dependent changes during acquisition of invasiveness also likely contribute to the ability of disseminating tumor cells to intravasate, survive and migrate in the circulation before extravasating at secondary site. At the secondary site, autophagy is required to maintain...
We tested the effect of autophagy inhibition on D2A1 cells seeded on BME matrices before and after their dormant-to-proliferative switch which occurs after 4–6 days of culture8 (Supplementary Fig. 1). We observed a significant increase in the percentage of dead cells when HCQ was added imm...
In cynomolgus monkey brains, autophagy level significantly diminishes before amyloid plaque formation, showing that autophagy inhibition is an important step for neurological disease development in primates [114]. A new experiment has used adult Macaca mulatta for autophagy activation status and its role ...
Subsequent processing is the same as before. 2.2. Biochemical parameters of plasma Blood samples were collected from the retroorbital sinus under anesthesia. Plasma was obtained after 3000 rpm centrifugation for 15 min at 4 °C. The levels of BUN, Cre, Ca2+ and Pi in plasma were ...
All the experimental mice were weighed and analysed survival rates (Fig. 1a) to assess their general conditions (Fig. 1b). After the induction of the ICH model, both the ICH and BYHWD groups showed a decrease in weight growth rate compared to the sham group. However, after drug interventi...
Aut1 loss of function mutant larvae fail to induce autophagy in the fat body before puparium stage and die during metamorphosis [26]. Recently, SNF4Aγ function in autophagosome formation during larval metamorphosis was revealed through a combination of Drosophila mutational and RNAi studies [28]....
of casein: whey protein 1:1 (w/w) by gavage immediately after exercise), exercise and supplementation 1 h after exercise (EXE/Suppl-1h; after fast, rats were submitted to the resistance protocol and received a mix of casein: whey protein 1:1 (w/w) by gavage 1 h after exercise). In...
cells Review Multiscale and Multimodal Approaches to Study Autophagy in Model Plants Jessica Marion 1, Romain Le Bars 1 ID , Laetitia Besse 1, Henri Batoko 2,* ID and Béatrice Satiat-Jeunemaitre 1 1 Institute of Integrative Biology of the Cell (I2BC), CEA, CNRS, Université Paris-Sud,...
Both Canonical and alternative autophagy must form double-membraned vacuoles, called autophagosomes, to sequester substrates before delivering it to the lysosome for degradation [7, 9]. Canonical autophagy mainly targets and degrades most long-lived proteins, big protein complexes and intracellular organ...
PDA specimens and adjacent non-tumor tissues were obtained from patients undergoing surgery at Sun Yat-sen Memorial Hospital. All specimens were derived from patients who had not undergone chemotherapy or radiotherapy before surgery. Patient clinicopathological characteristics are summarized in Additional file...