it is much more similar to a dimmer that gradually illuminates as your fast duration increases past 16 hours of fasting. According to clinicalresearch, your fasting autophagy dimmer switch is not at peak “brightness” until at least 36–72...
36. Grove, M. & Plumb, M. C/EBP, NF-kappa B, and c-Ets family members and transcriptional regulation of the cell-specific and inducible macrophage inflammatory protein 1 alpha immediate-early gene. Mol. Cell. Biol. 13, 5276–5289 (1993). 37. Zhang, Z. et al. CCAAT/enhancer-binding...
Importantly, this acceleration by ND was best revealed under conditions of BAF treatment, reflect- ing the previously reported fast turnover of formed autophagosomes (i.e. autophagic flux) [35]. The addition of BAF increased the vesicle size as well as the vesicle count of lysosomes, most ...
Subsequently, we profiled the dynamic changes of m6A in mRNAs during nutrient deficiency. Consistent with published studies36, m6A peaks were significantly enriched with the ‘GGAC’ motif and predominantly located in the CDS and 3′UTR regions, especially near the stop codon, in both control and...
The samples were then re-incubated at 37 °C for an additional hour. For this phase, we followed the manufacturer’s instructions to treat the slices with anti-Beclin 1 (MA5-15,825; Invitrogen, USA, 1:200), anti-LC3 (ab192890; Abcam, Cambridge, UK, 1:2000), and anti-p62 (ab9...
We thank the Center for Advanced Imaging (CAi) at the University Düsseldorf for providing access to the Zeiss LSM 880 Airyscan Fast. We are grateful to Reinhard Jahn and Alex Stein for continuous advice over the course of this project, and access to the DLS equipment. This work was ...
S3). Although this upregulation might appear fast considering a reported maturation half-time of more than 30 min (41), other studies have shown faster CP maturation (42). The reduction in proteasome levels at later time points was accompanied by an increase in a faster-migrating GFP species...
Ramping up your intermittent fasting and doing a 16-18 hour daily fast with a weekly 24-48 hour fast can also be a great weekly stimulator of autophagy to keep the body cleaned up. The key with intermittent fasting is that when you are in your eating window, you want to eat well and...
Fast protein liquid chromatography was performed to obtain high titer viral stocks (titers: 2–4 × 108 TU/μl). The virus stocks were then tested on primary cortical neurons for transduction efficacy and toxicity and viral titers were determined using qPCR. PMN culture, nucleofection and viral ...
mRNA expression was tested by using the SYBR GREEN PCR Master Mix (Applied Biosystems, Beijing, China) under a 7500 fast Real-time PCR System (Applied Biosystems). Relative expression of targeted mRNAs was calculated by the 2−ΔΔCt method, using β-actin as the internal control. All the...