Melatonin is a hormone secreted by the pineal gland, usually related to protection against oxidative stress due to its antioxidant properties23. The rate of melatonin secretion varies synchronously with the vaginal cycle, being higher when the oestrogen levels are lower at dioestrus24. It has been ...
we analyzed whether YTHDF3 regulates autophagy flux by simultaneously affecting lysosomal activity. Acridine orange (AO) is a cell-permeant dye that emits green fluorescence; however, when trapped in acidic compartments, AO emits red fluorescence when excited by...
Abbreviations : 3-MA: 3-methyladenine; ATG: autophagy-related gene; Cer: ceramide; CL: cardiolipin; Con A: concanamycin A; DAG: diradylglycerol; GO: gene ontology; HAG: hour after germination; LC-MS: liquid chromatography-mass spectrometry; MAG: min after germination; MDC: mono...
Although these data are updated every 24 hours, there may be a 48-hour delay before the most recent numbers are available. Preview View full text Download full text Access Options Alert me TOC email alert TOC RSS feed Citation email alert Citation RSS feed Abstract A major challenge...
Characterizing 24-Hour Skeletal Muscle Gene Expression Alongside Metabolic and Endocrine Responses Under Diurnal Conditions CONTEXT. Skeletal muscle plays a central role in the storage, synthesis, and breakdown of nutrients, yet little research has explored temporal responses of... HA Smith,T Iain,D ...
The MBPATG13HORMA-101 complex, amphipol-reconstituted MBPATG9, and amphipol-reconstituted MBPATG9-MBP13HORMA-101 complex, were crosslinked by incubating for 1 hour at 4 °C with 0.01% glutaraldehyde. The crosslinked samples were then quenched by adding 50 mM Tris pH8 and incubating for 15 ...
F Arbogast,R Sal-Carro,W Boufenghour,... - 《Biorxiv》 被引量: 0发表: 2022年 Lysosomotropic beta blockers induce oxidative stress and IL23A production in Langerhans cells Oxidative stress and T(h)17 cytokines are important mediators of inflammation. Treatment with beta-adrenoceptor (ADRB) anta...
Six-hour time-restricted feeding inhibits lung cancer progression and reshapes circadian metabolism Background: Accumulating evidence has suggested an oncogenic effect of diurnal disruption on cancer progression. To test whether targeting circadian rhythm... D Shi,G Fang,Q Chen,... - 《Bmc Medicine...
The microplates are kept for 72 hour at 37 °C in a humidified atmosphere containing 5% CO2. The adherent human cell line HeLa is cultured in MEM Eagle with 100 units/mL penicillin/100 μg/mL streptomycin, 10% FBS, and 2 mM l-glutamine in vented culture flasks. The adherent cells are...
24-hour urinary specimens were collected to determine albumin and creatinine levels for calculation of urinary albumin / creatinine ratio( UACR),and the kidneys were taken and weighted to calculate kidney weight/body weight ratio( KW/BW).Renal pathological changes were observed by PAS staining.Size...