their cells were more ready to use autophagy to recycle cell parts than when they were on their baseline schedule. Since the stress was rather mild and short—just an 18-hour fast for 4 days—the findings suggest
Autophagy fasting is a dietary strategy that involves fasting for a specific period of time to stimulate autophagy. During a fast, once your body has burned through much of its stored carbohydrate energy resulting in low liver glycogen levels, you enter a state of ketosis, where fats are broken...
Glutathione Sepharose beads bound with GST, GST-Rab39A, or GST-Rab39B were incubated with purified VPS39-Flag or VPS41-Flag for one hour, and then washed and boiled for immunoblotting. Source data are provided as a Source Data file. Full size image Next, we examined if the HOPS complex...
we found that levels of m6A reader YTHDF3 significantly increased (Fig.1a). We used a western blot to verify this up-regulation. During nutrient deprivation, levels of YTHDF3 greatly increased
S3). Although this upregulation might appear fast considering a reported maturation half-time of more than 30 min (41), other studies have shown faster CP maturation (42). The reduction in proteasome levels at later time points was accompanied by an increase in a faster-migrating GFP species...
We thank the Center for Advanced Imaging (CAi) at the University Düsseldorf for providing access to the Zeiss LSM 880 Airyscan Fast. We are grateful to Reinhard Jahn and Alex Stein for continuous advice over the course of this project, and access to the DLS equipment. This work was ...
following an overnight fast to induce diabetes [40]. For 48 h, the rats were given sucrose to avoid their deaths from the rise in insulin. After 72 h, a Glucometer was used to assess glucose levels in blood samples collected from the tail veins of the rats. Only diabetic rats (...
Data are presented as microlitres of food per fly per hour. Fly polyamine measurement Twenty flies (wet weight of snap-frozen fly bodies was estimated and used for normalization) were placed into 1.5-ml LoBind reaction tubes (Eppendorf, 0030108116), snap-frozen in liquid nitrogen and ...
the open field test, and their behavior digitally recorded for 20 min (habituation period) following which they were injected with amphetamine (ip, 1.5 mg/kg) and placed back in the center of the same transparent Plexiglas box and further monitored by videotaping for an additional hour. ...
Fast protein liquid chromatography was performed to obtain high titer viral stocks (titers: 2–4 × 108 TU/μl). The virus stocks were then tested on primary cortical neurons for transduction efficacy and toxicity and viral titers were determined using qPCR. PMN culture, nucleofection and viral ...