Knockdown of the combinational SNARE proteins VAMP8 and Vti1b with siRNAs disturbed the autophagic fusion of xenophagosomes with lysosomes, and cellular bactericidal efficiency significantly diminished. Furthermore, knockdown of these SNAREs inhibited the fusion of canonical autophagosomes with lysosomes. ...
Central role of mitofusin 2 in autophagosome-lysosome fusion in cardiomyocytes In the heart, autophagy has been implicated in car Zhao,Huang,Han,... - 《Journal of Biological Chemistry》 被引量: 117发表: 2012年 Autophagosome–lysosome fusion is independent of V-ATPase-mediated acidification The AT...
(4,5)P2, an inhibitor of the calcium channel mucolipin-1 that controls autophagosome–lysosome fusion. Hence, autophagosomes accumulate in OCRL-depleted cells and in the kidneys of Lowe syndrome patients. Importantly, boosting the activity of mucolipin-1 with selective agonists restores the ...
The ATP-dependent proton pump V-ATPase ensures low intralysosomal pH, which is essential for lysosomal hydrolase activity. Based on studies with the V-ATPase inhibitor BafilomycinA1, lysosomal acidification is also thought to be required for fusion with
DIPK2A promotes STX17- and VAMP7-mediated autophagosome-lysosome fusion by binding to VAMP7B 自噬体与溶酶体的融合是一个复杂的过程,本篇文章所做的研究旨在证明DIPK2A通过与STX17竞争结合VAMP7B,从而促进STX17与VAMP7A结合。进而使自噬体溶酶体融合。此外,DIPK2A还可通过促进自噬,抑制细胞凋亡。
homotypic fusion and protein sorting; IB, immu- noblotting; KO, knockout; LAMP1, lysosome-associated membrane protein 1; LRO, lysosome-related organelle; MAP1LC3/LC3, microtubule-associated protein 1 light chain 3; MEF, mouse embryonic fibroblast; RFP, red fluorescent protein; SNARE, soluble N...
In this punctum, we discuss how the C9orf72-RAB39A-HOPS axis function regulates autophagosome-lysosome fusion. 展开 关键词: Autophagosome-lysosome fusion C9orf72 HOPS complex membrane tethering RAB39A DOI: 10.1080/15548627.2023.2291938 年份: 2024 ...
Vacuole formation depended on autophagy and was modulated by a suppressor and inducer of autophagosome-lysosome fusion. Moreover, PGRN, a regulator of autophagy, was required for vacuolization induced by Abe or Vac. These results demonstrated the potential of Abe and Vac as novel tools for ...
Inhibition of SCD1 impairs palmitate-derived autophagy at the step of autophagosome-lysosome fusion in pancreatic beta-cells et al. Inhibition of SCD1 impairs palmitate-derived autophagy at the step of autophagosome-lysosome fusion in pancreatic β-cells. J. Lipid Res. 56, ... Janikiewicz,...
Autophagosome-lysosome fusion mediated by SNARE complexes is an essential step in autophagy.Two SNAP29-containing SNARE complexes have been extensively studied in starvation-induced bulk autophagy,while the relevant SNARE complexes in other types of autophagy occurring under non-starvation conditions have ...