Multiple Small GTPases Promote Autophagosome-Lysosome Fusion Motor Proteins Ensure That Autophagosomes Encounter Lysosomes Tethers and Adaptors Anchor Autophagosomes to Endosomes or Lysosomes SNARE Proteins Execute the Fusion of Autophagosomes with Lysosomes The Lipid Composition of the Autophagosome Regulates ...
Autophagosome-Lysosome Fusion Depends on the pH in Acidic Compartments in CHO Cells Autophagy is the bulk degradation of cytoplasmic constituents in response to starvation and other environmental or intracellular cues. During this process,... A Kawai,H Uchiyama,S Takano,... - 《Autophagy》 被引量...
自噬溶酶体(autolysosome)又称胞溶酶体(cytolysosome),它是初级溶酶体与来自自噬作用(autophagocytosis)的含有内源性物质的囊泡融合而成。内源性物质包括细胞内由于生理或病理原因而被损伤的细胞器,或过量储存的糖元等,它们可被细胞自身的膜(如内质网或高尔基复合体的膜)包裹形成自噬体(autophgosome),自噬体与初级溶...
V-ATPase is not required for autophagosome–lysosome fusion BafA1, a chemical inhibitor of the V-ATPase, is commonly used to block autophagosome–lysosome fusion in mammalian cell culture studies, and causes an accumulation of autophagosomes upon autophagy induction14,24. This phenomenon is reversible...
(4,5)P2, an inhibitor of the calcium channel mucolipin-1 that controls autophagosome–lysosome fusion. Hence, autophagosomes accumulate in OCRL-depleted cells and in the kidneys of Lowe syndrome patients. Importantly, boosting the activity of mucolipin-1 with selective agonists restores the ...
[1] Tian X, Zheng P, Zhou C, Wang X, Ma H, Ma W, Zhou X, Teng J, Chen J. DIPK2A promotes STX17- and VAMP7-mediated autophagosome-lysosome fusion by binding to VAMP7B. Autophagy. 2020 May;16(5):797-810. doi: 10.1080/15548627.2019.1637199. Epub 2019 Jul 4. PMID: 31251111;...
homotypic fusion and protein sorting; IB, immu- noblotting; KO, knockout; LAMP1, lysosome-associated membrane protein 1; LRO, lysosome-related organelle; MAP1LC3/LC3, microtubule-associated protein 1 light chain 3; MEF, mouse embryonic fibroblast; RFP, red fluorescent protein; SNARE, soluble N...
Moreover, vacuole formation was inhibited by the knockdown of progranulin (PGRN), a regulator of autophagosome-lysosome fusion, and promoted by its overexpression. The present results suggest the potential of Abe-/Vac-induced vacuole-like autolysosomes as a tool for evaluating autophagosome-lysosome ...
Mechanism of cellular authophagy illustration for Nobel Prize Award in Medicine 2016. 3D illustration showing fusion of lysosome with autophagosome containing microbes and molecules,站酷海洛,一站式正版视觉内容平台,站酷旗下品牌.授权内容包含正版商业图片、
In contrast, BafilomycinA1 prevents autophagosome-lysosome fusion in these cells, and this defect is phenocopied by depletion of the Capump SERCA, a secondary target of this drug. Moreover, activation of SERCA promotes fusion in a BafilomycinA1-sensitive manner. Collectively, our results indicate ...