Interaction of epidermal growth factor-dependent protein kinase with endogenous membrane proteins and soluble peptide substrate We have studied the relationship between these EGF-stimulated activities using both [γ- 32P]ATP and adenosine 5'-O-(3-[ 35S]thiotriphosphate) ([ 35S]ATPγS) as phosphate...
It is concluded that the chelate structure of metal ATP substrate in the phosphomevalonate kinase reaction is the delta, beta, gamma-bidentate complex. 31P NMR measurements and radioassay with [2-14C] phosphomevalonate were used to measure the equilibrium of the reaction catalyzed by phospho...
GTP, as well as ATP, can act as a substrate for the intrinsic protein kinase activity of synaptic plasma membranes 来自 国家科技图书文献中心 喜欢 0 阅读量: 31 作者:M Weller,M Haaga,W Laing 摘要: GTP as well as ATP can act as phosphate donor for the intrinsic protein kinase activity of...
The kinase-mediated transfer of a ‘heavy’ 18O-labelled γ-phosphate group to a protein substrate subsequently marks newly-introduced phospho-moieties9,10. The advantages of such ATP analogues have been previously demonstrated, e.g., detection of phosphorylation sites9; determining specificities of ...
To predict the labeled peptides for all the protein kinases in Arabidopsis, we retrieved protein sequences of all protein kinases from the Arabidopsis thaliana kinase database. Sequences for each subfamily in the AthKD database were retrieved and aligned using MultiAlign. The labeled peptide that ...
Hill has challenged biochemists to find whether the heat of shortening of muscle has any equivalent in the chemistry of muscle. It is shown that the heat of shortening, which is reabsorbed as tension disappears, is not degraded free energy from the splitting of ATP. It must be reversibly ...
8、底物溶液(TMB Substrate):1×10ml/瓶。 9、浓洗涤液(Wash Buffer):1×20ml/瓶,使用时每瓶用蒸馏水稀释25倍。 10、终止液(Stop Solution):1×10ml/瓶(2N H2SO4)。 谷胱甘肽S转移酶θ2(GSTθ2)ELISA试剂盒GOLT1AGSTθ2 ELISA Kit高尔基体转运蛋白1A抗体 ...
The ability to use gamma((18)O4)-ATP to monitor phosphorylation modification within various proteins was conducted by performing in vitro kinase reactions in the presence of a 1:1 mixture of gamma((18)O4)-ATP and normal isotopic abundance ATP (ATP). After tryptic digestion, the peptides ...
Recent 26S cryo-electron microscopy (EM) analyses revealed that large conformational changes occur in the 19S when the ATPases bind ATPgS or substrates5–7. These similar ATP-bound and substrate-bound conformations are thought to be degradation competent. ATP (but not adenosine diphosphate (ADP...
8. 底物溶液(TMB Substrate):1×10ml/瓶。 9. 浓洗涤液(Wash Buffer):1×20ml/瓶,使用时每瓶用蒸馏水稀释25倍。 10. 终止液(Stop Solution):1×10ml/瓶(2N H2SO4)。 测定步骤: 1.加样 1. 除包被外都需45度加样 2.加样体积要准确 3.管底加样,不能加在管壁上 ...