Medical Financial Acronyms Encyclopedia Wikipedia glob·u·lin (glŏb′yə-lĭn) n. 1.Any of a class of proteins that are widespread in blood plasma, milk, muscle, and plant seeds and that are insoluble in pure water but soluble in dilute salt solutions. Blood serum globulins are divid...
71 In addition, the ATG16L1 (T300A) mutation increases susceptibility to caspase-3 processing of ATG16L1, leading to decreased levels of the full-length protein following dysfunctional autophagy.71 Chronic obstructive pulmonary disease (COPD) is a chronic inflammatory disease of mucosal tissues in ...
Survey full scan MS spectra were acquired in the orbitrap (m/z 500–1700) with a resolution of 120,000 and an automatic gain control (AGC) target at 500,000. The top 20 most intense ions were selected for HCD MS/MS fragmentation in the orbitrap at an AGC target of 30,000 and with...
and plays an essential role in the initiation of autophagy23. Next, we sought to determine the functions of LD targeting by ATG14. Knockdown of ATG14 caused the accumulation of LDs in untreated cells (Fig.1i). Overexpression of ATG14, but not ATG14WFY-RRR, led to the decreased LD numbe...
The similar results were obtained in additional two independent experiments. Full size image ATG therapy drives more CD62L- memory type Tregs We demonstrated (Figure 2) that ATG preferentially depletes CD62L+ naïve T cells. It was then of interest to learn whether ATG therapy depleted ...
Full size image To date, there have been several reports on the direct membrane association of the ULK/Atg1 complex components. A putative lipid-binding domain has been identified in ULK/Atg1 proteins. The C-terminus of ULK1 associates with cell membranes (Fig.2a, red line)37, and in line...
Microtubule-associated protein light chain 3 (LC3/ATG8) is converted to a membrane conjugated form by ATG4, while ATG9 is an integral membrane protein required for the formation of the double membrane vesicle [2], [10], [11]. ATG6/Beclin-1 functions in cooperation with the class ||| ...
Full size image ATG9A participates in the early stages of autophagy, presumably by delivering lipids to phagophore assembly sites (PAS) [51,52]. This function of ATG9A promotes the conversion of the autophagy protein LC3 (orthologous to yeast Atg8) from a soluble, cytosolic form (LC3-I) to...
Full size image Fig. 7: Impact of the Y8A mutation of ATG9A on its sorting and segregation from synaptophysin. Full size image Surprisingly, when ATG9AY8A-EGFP was expressed with both mCherry-synapsin and synaptophysin, it was not retained in the TGN and precisely colocalized with synaptophys...
Full size image Similarly, we sought to address whether SETD2 has an impact on ATG14 protein levels. For this purpose, immunoblot analyses of ATG14 performed in HeLa cells allowed the detection under control conditions of two distinct protein bands that upon BafA1 treatment led to the accumulat...